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Assay Detail

CHEMBL1810165

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Binding
Inhibition of GST-tagged FLT3 expressing wild type kinase domain expressed in Sf9 insect cells after 4 hrs by Kinase-Glo assay
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type Sf9
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Receptor-type tyrosine-protein kinase FLT3 (CHEMBL1974)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and evaluation of 3-phenyl-1H-5-pyrazolylamine-based derivatives as potent, selective and efficacious inhibitors of FMS-like tyrosine kinase-3 (FLT3).
Lin WH, Hsieh SY, Yen SC, Chen CT, Yeh TK, Hsu T, Lu CT, Chen CP, Chen CW, Chou LH, Huang YL, Cheng AH, Chang YI, Tseng YJ, Yen KR, Chao YS, Hsu JT, Jiaang WT.
Bioorg Med Chem (2011) 19 : 4173 -4182
PubMed 21708468 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 7.35 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1807483 1 8.22
CHEMBL1807478 1 8.15
CHEMBL1807469 1 8.15
CHEMBL1807467 1 8.10
CHEMBL1807789 1 8.00
CHEMBL1807785 1 8.00
CHEMBL1807392 1 7.89
CHEMBL1807386 1 7.85
CHEMBL1807470 1 7.82
CHEMBL223360 LINIFANIB 3.0 1 7.70
CHEMBL1807482 1 7.70
CHEMBL1807477 1 7.54
CHEMBL1807786 1 7.30
CHEMBL1336 SORAFENIB 4.0 1 7.27
CHEMBL1807788 1 7.22
CHEMBL1807475 1 7.19
CHEMBL1807479 1 7.16
CHEMBL1807787 1 7.15
CHEMBL1807471 1 7.05
CHEMBL1807473 1 7.00
CHEMBL1807485 1 6.96
CHEMBL1807468 1 6.92
CHEMBL1807484 1 6.92
CHEMBL1807474 1 6.80
CHEMBL1807481 1 6.57
CHEMBL1807480 1 6.46
CHEMBL1807472 1 6.44
CHEMBL1807466 1 6.23
CHEMBL1807389 1 -
CHEMBL1807388 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1807483 IC50 = 6.0 nM 8.22
CHEMBL1807478 IC50 = 7.0 nM 8.15
CHEMBL1807469 IC50 = 7.0 nM 8.15
CHEMBL1807467 IC50 = 8.0 nM 8.10
CHEMBL1807789 IC50 = 10.0 nM 8.00
CHEMBL1807785 IC50 = 10.0 nM 8.00
CHEMBL1807392 IC50 = 13.0 nM 7.89
CHEMBL1807386 IC50 = 14.0 nM 7.85
CHEMBL1807470 IC50 = 15.0 nM 7.82
CHEMBL223360 LINIFANIB IC50 = 20.0 nM 7.70
CHEMBL1807482 IC50 = 20.0 nM 7.70
CHEMBL1807477 IC50 = 29.0 nM 7.54
CHEMBL1807786 IC50 = 50.0 nM 7.30
CHEMBL1336 SORAFENIB IC50 = 54.0 nM 7.27
CHEMBL1807788 IC50 = 60.0 nM 7.22
CHEMBL1807475 IC50 = 65.0 nM 7.19
CHEMBL1807479 IC50 = 70.0 nM 7.16
CHEMBL1807787 IC50 = 71.0 nM 7.15
CHEMBL1807471 IC50 = 90.0 nM 7.05
CHEMBL1807473 IC50 = 100.0 nM 7.00
CHEMBL1807485 IC50 = 110.0 nM 6.96
CHEMBL1807468 IC50 = 120.0 nM 6.92
CHEMBL1807484 IC50 = 120.0 nM 6.92
CHEMBL1807474 IC50 = 160.0 nM 6.80
CHEMBL1807481 IC50 = 270.0 nM 6.57
CHEMBL1807480 IC50 = 350.0 nM 6.46
CHEMBL1807472 IC50 = 360.0 nM 6.44
CHEMBL1807466 IC50 = 590.0 nM 6.23
CHEMBL1807389 IC50 > 1000.0 nM -
CHEMBL1807388 IC50 > 1000.0 nM -
CHEMBL1807476 IC50 > 1000.0 nM -