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Assay Detail

CHEMBL1811340

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]-sauvagine from rat CRF-1 receptor expressed in human IMR-32 cells after 2 hrs by scintillation counting
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type IMR-32
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Corticotropin-releasing factor receptor 1 (CHEMBL4649)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Discovery of N-(1-ethylpropyl)-[3-methoxy-5-(2-methoxy-4-trifluoromethoxyphenyl)-6-methyl-pyrazin-2-yl]amine 59 (NGD 98-2): an orally active corticotropin releasing factor-1 (CRF-1) receptor antagonist.
Hodgetts KJ, Ge P, Yoon T, De Lombaert S, Brodbeck R, Gulianello M, Kieltyka A, Horvath RF, Kehne JH, Krause JE, Maynard GD, Hoffman D, Lee Y, Fung L, Doller D.
J Med Chem (2011) 54 : 4187 -4206
PubMed 21618986 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 8.26 8.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL475306 1 8.38
CHEMBL270948 1 8.29
CHEMBL482950 PEXACERFONT 3.0 1 8.21
CHEMBL270738 1 8.17

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL475306 IC50 = 4.2 nM 8.38
CHEMBL270948 IC50 = 5.1 nM 8.29
CHEMBL482950 PEXACERFONT IC50 = 6.1 nM 8.21
CHEMBL270738 IC50 = 6.8 nM 8.17