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Assay Detail

CHEMBL1815135

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Transactivation of human PPARalpha expressed in human HepG2 cells co-transfected with PPRE3-TK-Luc by luciferase reporter gene assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HepG2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor alpha (CHEMBL239)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Revisiting glitazars: thiophene substituted oxazole containing α-ethoxy phenylpropanoic acid derivatives as highly potent PPARα/γ dual agonists devoid of adverse effects in rodents.
Raval P, Jain M, Goswami A, Basu S, Gite A, Godha A, Pingali H, Raval S, Giri S, Suthar D, Shah M, Patel P.
Bioorg Med Chem Lett (2011) 21 : 3103 -3109
PubMed 21450468 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 12 8.36 10.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1813003 1 10.59
CHEMBL1813008 1 10.00
CHEMBL1813009 1 8.72
CHEMBL1813004 1 8.52
CHEMBL1813007 1 7.70
CHEMBL1813012 1 7.66
CHEMBL295416 PIRINIXIC ACID 2.0 1 5.32
CHEMBL1813005 1 -
CHEMBL1813006 1 -
CHEMBL1813010 1 -
CHEMBL1813011 1 -
CHEMBL121 ROSIGLITAZONE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1813003 EC50 = 0.026 nM 10.59
CHEMBL1813008 EC50 = 0.1 nM 10.00
CHEMBL1813009 EC50 = 1.9 nM 8.72
CHEMBL1813004 EC50 = 3.0 nM 8.52
CHEMBL1813007 EC50 = 20.0 nM 7.70
CHEMBL1813012 EC50 = 22.0 nM 7.66
CHEMBL295416 PIRINIXIC ACID EC50 = 4800.0 nM 5.32
CHEMBL1813005 EC50 = 6e-05 nM -
CHEMBL1813006 EC50 = 5e-05 nM -
CHEMBL1813010 EC50 = 7e-05 nM -
CHEMBL1813011 EC50 = 0.00031 nM -
CHEMBL121 ROSIGLITAZONE EC50 - -