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Assay Detail

CHEMBL1815453

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human DAT expressed in HEK293 cells assessed as inhibition of [3H]DA reuptake after 10 mins by scintillation counting
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium-dependent dopamine transporter (CHEMBL238)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Nicotinic acetylcholine receptor efficacy and pharmacological properties of 3-(substituted phenyl)-2β-substituted tropanes.
Carroll FI, Blough BE, Mascarella SW, Navarro HA, Eaton JB, Lukas RJ, Damaj MI.
J Med Chem (2010) 53 : 8345 -8353
PubMed 21058665 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 8.30 9.12

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1812741 1 9.12
CHEMBL1812739 1 9.08
CHEMBL1812747 1 8.82
CHEMBL1812745 1 8.70
CHEMBL1812746 1 8.64
CHEMBL1812749 1 8.52
CHEMBL1812743 1 8.41
CHEMBL1812740 1 8.32
CHEMBL1812750 1 8.19
CHEMBL1812744 1 8.14
CHEMBL1812742 1 7.96
CHEMBL1812748 1 7.82
CHEMBL894 BUPROPION 4.0 1 6.18

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1812741 IC50 = 0.75 nM 9.12
CHEMBL1812739 IC50 = 0.83 nM 9.08
CHEMBL1812747 IC50 = 1.5 nM 8.82
CHEMBL1812745 IC50 = 2.0 nM 8.70
CHEMBL1812746 IC50 = 2.3 nM 8.64
CHEMBL1812749 IC50 = 3.0 nM 8.52
CHEMBL1812743 IC50 = 3.9 nM 8.41
CHEMBL1812740 IC50 = 4.8 nM 8.32
CHEMBL1812750 IC50 = 6.4 nM 8.19
CHEMBL1812744 IC50 = 7.3 nM 8.14
CHEMBL1812742 IC50 = 11.0 nM 7.96
CHEMBL1812748 IC50 = 15.0 nM 7.82
CHEMBL894 BUPROPION IC50 = 658.0 nM 6.18