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Assay Detail

CHEMBL1817224

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to CFTR F508 deletion mutant expressed in forskolin-stimulated FRT cells assessed as increase in iodine influx measured as YFP quenching rate after 24 hrs by fluorescence assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type FRT
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Cystic fibrosis: a new target for 4-Imidazo[2,1-b]thiazole-1,4-dihydropyridines.
Budriesi R, Ioan P, Leoni A, Pedemonte N, Locatelli A, Micucci M, Chiarini A, Galietta LJ.
J Med Chem (2011) 54 : 3885 -3894
PubMed 21568323 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 23 5.41 6.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1814700 1 6.77
CHEMBL1814576 1 6.75
CHEMBL1814577 1 6.50
CHEMBL1814699 1 6.43
CHEMBL1814701 1 6.40
CHEMBL272211 1 5.85
CHEMBL411450 1 5.55
CHEMBL193 NIFEDIPINE 4.0 1 5.54
CHEMBL1814575 1 5.50
CHEMBL1814584 1 5.50
CHEMBL1814578 1 5.46
CHEMBL1814579 1 5.32
CHEMBL271969 1 5.17
CHEMBL1814583 1 5.08
CHEMBL44 GENISTEIN 2.0 1 5.03
CHEMBL410241 1 4.96
CHEMBL272810 1 4.92
CHEMBL1814580 1 4.83
CHEMBL1814574 1 4.71
CHEMBL270674 1 4.67
CHEMBL272472 1 4.55
CHEMBL411235 1 4.47
CHEMBL271970 1 4.37

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1814700 Kd = 170.0 nM 6.77
CHEMBL1814576 Kd = 180.0 nM 6.75
CHEMBL1814577 Kd = 320.0 nM 6.50
CHEMBL1814699 Kd = 370.0 nM 6.43
CHEMBL1814701 Kd = 400.0 nM 6.40
CHEMBL272211 Kd = 1400.0 nM 5.85
CHEMBL411450 Kd = 2800.0 nM 5.55
CHEMBL193 NIFEDIPINE Kd = 2900.0 nM 5.54
CHEMBL1814575 Kd = 3200.0 nM 5.50
CHEMBL1814584 Kd = 3200.0 nM 5.50
CHEMBL1814578 Kd = 3500.0 nM 5.46
CHEMBL1814579 Kd = 4800.0 nM 5.32
CHEMBL271969 Kd = 6800.0 nM 5.17
CHEMBL1814583 Kd = 8400.0 nM 5.08
CHEMBL44 GENISTEIN Kd = 9400.0 nM 5.03
CHEMBL410241 Kd = 10900.0 nM 4.96
CHEMBL272810 Kd = 12000.0 nM 4.92
CHEMBL1814580 Kd = 14800.0 nM 4.83
CHEMBL1814574 Kd = 19400.0 nM 4.71
CHEMBL270674 Kd = 21400.0 nM 4.67
CHEMBL272472 Kd = 28300.0 nM 4.55
CHEMBL411235 Kd = 34200.0 nM 4.47
CHEMBL271970 Kd = 42900.0 nM 4.37