Assay Detail
Functional
CHEMBL1820156
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at rat TRPA1 channel expressed in HEK293 cells assessed as calcium influx measured at every 1 second for 60 seconds by fluorescence analysis
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Rattus norvegicus |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Transient receptor potential cation channel subfamily A member 1 (CHEMBL5160) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
Design and pharmacological evaluation of PF-4840154, a non-electrophilic reference agonist of the TrpA1 channel.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 10 | 6.20 | 7.01 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1818218 | — | — | 1 | 7.01 |
| CHEMBL1818209 | — | — | 1 | 6.76 |
| CHEMBL1818214 | — | — | 1 | 6.73 |
| CHEMBL1818210 | — | — | 1 | 6.67 |
| CHEMBL1818215 | — | — | 1 | 6.33 |
| CHEMBL1818212 | — | — | 1 | 6.24 |
| CHEMBL1818217 | — | — | 1 | 6.07 |
| CHEMBL1818211 | — | — | 1 | 5.65 |
| CHEMBL1818213 | — | — | 1 | 5.25 |
| CHEMBL233248 | ALLYL ISOTHIOCYANATE | 1.0 | 1 | 5.24 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1818218 | — | EC50 | = | 97.0 | nM | 7.01 |
| CHEMBL1818209 | — | EC50 | = | 175.0 | nM | 6.76 |
| CHEMBL1818214 | — | EC50 | = | 186.0 | nM | 6.73 |
| CHEMBL1818210 | — | EC50 | = | 215.0 | nM | 6.67 |
| CHEMBL1818215 | — | EC50 | = | 468.0 | nM | 6.33 |
| CHEMBL1818212 | — | EC50 | = | 581.0 | nM | 6.24 |
| CHEMBL1818217 | — | EC50 | = | 848.0 | nM | 6.07 |
| CHEMBL1818211 | — | EC50 | = | 2250.0 | nM | 5.65 |
| CHEMBL1818213 | — | EC50 | = | 5600.0 | nM | 5.25 |
| CHEMBL233248 | ALLYL ISOTHIOCYANATE | EC50 | = | 5813.0 | nM | 5.24 |