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Assay Detail

CHEMBL1820765

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity in human NCI-H460 cells in serum free medium at 50 nM after 12 hrs by Hoechst 33342/propidium iodide staining based fluorescence microscopy
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type NCI-H460
Confidence 1 — Organism
Curated By Autocuration

Target

NCI-H460 (CHEMBL396)
Type CELL-LINE
Organism Homo sapiens

Publication

C5'-Alkyl Substitution Effects on Digitoxigenin α-l-Glycoside Cancer Cytotoxicity.
Wang HY, Qi Z, Wu B, Kang SW, Rojanasakul Y, O'Doherty GA.
ACS Med Chem Lett (2011) 2 : 259 -263
PubMed 21572583 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.89 7.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1651648 1 7.28
CHEMBL1651649 RAMNODIGIN 2.0 1 7.28
CHEMBL1818689 1 7.24
CHEMBL1818693 1 7.06
CHEMBL1818690 1 6.94
CHEMBL1818691 1 6.89
CHEMBL1818692 1 6.75
CHEMBL1818812 1 6.34
CHEMBL1818694 1 6.27

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1651648 IC50 = 52.0 nM 7.28
CHEMBL1651649 RAMNODIGIN IC50 = 52.0 nM 7.28
CHEMBL1818689 IC50 = 57.0 nM 7.24
CHEMBL1818693 IC50 = 87.0 nM 7.06
CHEMBL1818690 IC50 = 116.0 nM 6.94
CHEMBL1818691 IC50 = 130.0 nM 6.89
CHEMBL1818692 IC50 = 180.0 nM 6.75
CHEMBL1818812 IC50 = 458.0 nM 6.34
CHEMBL1818694 IC50 = 533.0 nM 6.27