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Assay Detail

CHEMBL1821018

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of DPP4 in human plasma assessed as formation of 7-amino-4-methylcoumarin from glycyl-L-proline 4-methylcoumaryl-7-amide by fluorescence assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Tissue Plasma
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dipeptidyl peptidase 4 (CHEMBL284)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

2-({6-[(3R)-3-amino-3-methylpiperidine-1-yl]-1,3-dimethyl-2,4-dioxo-1,2,3,4-tetrahydro-5H-pyrrolo[3,2-d]pyrimidine-5-yl}methyl)-4-fluorobenzonitrile (DSR-12727): a potent, orally active dipeptidyl peptidase IV inhibitor without mechanism-based inactivation of CYP3A.
Nishio Y, Kimura H, Sawada N, Sugaru E, Horiguchi M, Ono M, Furuta Y, Sakai M, Masui Y, Otani M, Hashizuka T, Honda Y, Deguchi J, Nakagawa T, Nakahira H.
Bioorg Med Chem (2011) 19 : 5490 -5499
PubMed 21865048 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 8.79 9.47

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1819089 1 9.47
CHEMBL142703 VILDAGLIPTIN 4.0 1 9.24
CHEMBL376359 ALOGLIPTIN 4.0 1 9.00
CHEMBL1819090 1 8.96
CHEMBL1422 SITAGLIPTIN 4.0 1 8.46
CHEMBL1819091 1 7.58
CHEMBL1819092 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1819089 IC50 = 0.34 nM 9.47
CHEMBL142703 VILDAGLIPTIN IC50 = 0.58 nM 9.24
CHEMBL376359 ALOGLIPTIN IC50 = 1.0 nM 9.00
CHEMBL1819090 IC50 = 1.1 nM 8.96
CHEMBL1422 SITAGLIPTIN IC50 = 3.5 nM 8.46
CHEMBL1819091 IC50 = 26.0 nM 7.58
CHEMBL1819092 IC50 > 1000.0 nM -