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Assay Detail

CHEMBL1821157

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Modulation of human PPARgamma-LBD expressed in african green monkey COS7 cells co-transfected with Gal4 assessed as activation of transactivation activity by luciferase assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type COS-7
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor gamma (CHEMBL235)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

SAR studies of acidic dual γ-secretase/PPARγ modulators.
Hieke M, Ness J, Steri R, Greiner C, Werz O, Schubert-Zsilavecz M, Weggen S, Zettl H.
Bioorg Med Chem (2011) 19 : 5372 -5382
PubMed 21873070 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 17 5.57 7.37

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL121 ROSIGLITAZONE 4.0 1 7.37
CHEMBL595 PIOGLITAZONE 4.0 1 6.52
CHEMBL1819481 1 5.96
CHEMBL1819483 1 5.82
CHEMBL1819397 1 5.75
CHEMBL1819394 1 5.66
CHEMBL1819482 1 5.60
CHEMBL1819392 1 5.47
CHEMBL1819393 1 5.43
CHEMBL1819390 1 5.33
CHEMBL1817687 1 5.32
CHEMBL1171834 1 5.18
CHEMBL1819478 1 5.14
CHEMBL1819391 1 5.11
CHEMBL461883 1 5.08
CHEMBL1819396 1 4.97
CHEMBL1170264 1 4.96

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL121 ROSIGLITAZONE EC50 = 43.0 nM 7.37
CHEMBL595 PIOGLITAZONE EC50 = 300.0 nM 6.52
CHEMBL1819481 EC50 = 1100.0 nM 5.96
CHEMBL1819483 EC50 = 1500.0 nM 5.82
CHEMBL1819397 EC50 = 1800.0 nM 5.75
CHEMBL1819394 EC50 = 2200.0 nM 5.66
CHEMBL1819482 EC50 = 2500.0 nM 5.60
CHEMBL1819392 EC50 = 3400.0 nM 5.47
CHEMBL1819393 EC50 = 3700.0 nM 5.43
CHEMBL1819390 EC50 = 4700.0 nM 5.33
CHEMBL1817687 EC50 = 4800.0 nM 5.32
CHEMBL1171834 EC50 = 6600.0 nM 5.18
CHEMBL1819478 EC50 = 7200.0 nM 5.14
CHEMBL1819391 EC50 = 7800.0 nM 5.11
CHEMBL461883 EC50 = 8300.0 nM 5.08
CHEMBL1819396 EC50 = 10800.0 nM 4.97
CHEMBL1170264 EC50 = 11000.0 nM 4.96