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Assay Detail

CHEMBL1831613

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of TGF-beta1 signaling in human HEK293 cells transfected with luciferase and FAST-2 gene expression vector A3-LUX after 16 hrs by luciferase reporter gene assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type HEK293
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

TGF-beta receptor type-1 (CHEMBL4439)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

2-Phenyl and 2-heterocyclic-4-(3-(pyridin-2-yl)-1H-pyrazol-4-yl)pyridines as inhibitors of TGF-β1 and activin A signalling.
Ciayadi R, Potdar M, Walton KL, Harrison CA, Kelso GF, Harris SJ, Hearn MT.
Bioorg Med Chem Lett (2011) 21 : 5642 -5645
PubMed 21783359 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 7.27 7.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1829511 1 7.96
CHEMBL424676 1 7.72
CHEMBL1829512 1 7.50
CHEMBL1828640 1 7.17
CHEMBL1829504 1 7.11
CHEMBL1829505 1 7.06
CHEMBL202887 1 6.35

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1829511 IC50 = 11.0 nM 7.96
CHEMBL424676 IC50 = 19.0 nM 7.72
CHEMBL1829512 IC50 = 32.0 nM 7.50
CHEMBL1828640 IC50 = 68.0 nM 7.17
CHEMBL1829504 IC50 = 77.0 nM 7.11
CHEMBL1829505 IC50 = 88.0 nM 7.06
CHEMBL202887 IC50 = 446.0 nM 6.35