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Assay Detail

CHEMBL1832525

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of HDAC6 using KI-104 as substrate by fluorescence assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Histone deacetylase 6 (CHEMBL1865)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of (2E)-3-{2-butyl-1-[2-(diethylamino)ethyl]-1H-benzimidazol-5-yl}-N-hydroxyacrylamide (SB939), an orally active histone deacetylase inhibitor with a superior preclinical profile.
Wang H, Yu N, Chen D, Lee KC, Lye PL, Chang JW, Deng W, Ng MC, Lu T, Khoo ML, Poulsen A, Sangthongpitag K, Wu X, Hu C, Goh KC, Wang X, Fang L, Goh KL, Khng HH, Goh SK, Yeo P, Liu X, Bonday Z, Wood JM, Dymock BW, Kantharaj E, Sun ET.
J Med Chem (2011) 54 : 4694 -4720
PubMed 21634430 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 14 7.32 9.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL483254 PANOBINOSTAT 4.0 1 9.15
CHEMBL1830536 1 8.40
CHEMBL408513 BELINOSTAT 4.0 1 8.00
CHEMBL491316 1 7.70
CHEMBL460963 1 7.64
CHEMBL489332 1 7.57
CHEMBL98 VORINOSTAT 4.0 1 7.54
CHEMBL1830397 1 7.03
CHEMBL1830396 1 6.65
CHEMBL1830537 1 6.64
CHEMBL3215861 1 6.61
CHEMBL1830420 1 6.55
CHEMBL1830424 1 6.52
CHEMBL1830422 1 6.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL483254 PANOBINOSTAT Ki = 0.7 nM 9.15
CHEMBL1830536 Ki = 4.0 nM 8.40
CHEMBL408513 BELINOSTAT Ki = 10.0 nM 8.00
CHEMBL491316 Ki = 20.0 nM 7.70
CHEMBL460963 Ki = 23.0 nM 7.64
CHEMBL489332 Ki = 27.0 nM 7.57
CHEMBL98 VORINOSTAT Ki = 29.0 nM 7.54
CHEMBL1830397 Ki = 94.0 nM 7.03
CHEMBL1830396 Ki = 225.0 nM 6.65
CHEMBL1830537 Ki = 227.0 nM 6.64
CHEMBL3215861 Ki = 247.0 nM 6.61
CHEMBL1830420 Ki = 282.0 nM 6.55
CHEMBL1830424 Ki = 300.0 nM 6.52
CHEMBL1830422 Ki = 348.0 nM 6.46