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Assay Detail

CHEMBL1833115

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant MAO-B expressed in baculovirus infected BTI-TN-5B1-4 cells assessed as production of hydrogen peroxide from p-tyramine after 15 mins by microplate fluorescence assay
35
Total Activities
35
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type BTI-TN-5B1-4
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Amine oxidase [flavin-containing] B (CHEMBL2039)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and selective human monoamine oxidase inhibition of 3-carbonyl, 3-acyl, and 3-carboxyhydrazido coumarin derivatives.
Secci D, Carradori S, Bolasco A, Chimenti P, Yáñez M, Ortuso F, Alcaro S.
Eur J Med Chem (2011) 46 : 4846 -4852
PubMed 21872365 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 35 5.52 8.49

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1830829 1 8.49
CHEMBL1830814 1 8.15
CHEMBL1830817 1 8.02
CHEMBL972 SELEGILINE 4.0 1 7.71
CHEMBL1830818 1 7.67
CHEMBL1830815 1 7.21
CHEMBL1830796 1 6.85
CHEMBL1830797 1 6.70
CHEMBL1830827 1 6.39
CHEMBL1830833 1 5.92
CHEMBL1830825 1 5.75
CHEMBL1501880 1 5.70
CHEMBL1830816 1 5.43
CHEMBL1830830 1 5.34
CHEMBL92401 IPRONIAZID 2.0 1 5.12
CHEMBL1830828 1 5.06
CHEMBL1830809 1 5.00
CHEMBL149463 3-ACETYLCOUMARIN 1 4.84
CHEMBL1830808 1 4.83
CHEMBL1411619 1 4.81
CHEMBL1830811 1 4.76
CHEMBL1830824 1 4.75
CHEMBL1830800 1 4.75
CHEMBL1830795 1 4.75
CHEMBL379350 1 4.74
CHEMBL326294 ISATIN 1 4.73
CHEMBL1830810 1 4.67
CHEMBL568385 1 4.63
CHEMBL1830801 1 4.62
CHEMBL1830799 1 4.57

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1830829 IC50 = 3.22 nM 8.49
CHEMBL1830814 IC50 = 7.01 nM 8.15
CHEMBL1830817 IC50 = 9.47 nM 8.02
CHEMBL972 SELEGILINE IC50 = 19.6 nM 7.71
CHEMBL1830818 IC50 = 21.47 nM 7.67
CHEMBL1830815 IC50 = 61.2 nM 7.21
CHEMBL1830796 IC50 = 140.0 nM 6.85
CHEMBL1830797 IC50 = 200.0 nM 6.70
CHEMBL1830827 IC50 = 410.0 nM 6.39
CHEMBL1830833 IC50 = 1190.0 nM 5.92
CHEMBL1830825 IC50 = 1800.0 nM 5.75
CHEMBL1501880 IC50 = 2010.0 nM 5.70
CHEMBL1830816 IC50 = 3750.0 nM 5.43
CHEMBL1830830 IC50 = 4520.0 nM 5.34
CHEMBL92401 IPRONIAZID IC50 = 7540.0 nM 5.12
CHEMBL1830828 IC50 = 8620.0 nM 5.06
CHEMBL1830809 IC50 = 10060.0 nM 5.00
CHEMBL149463 3-ACETYLCOUMARIN IC50 = 14450.0 nM 4.84
CHEMBL1830808 IC50 = 14810.0 nM 4.83
CHEMBL1411619 IC50 = 15400.0 nM 4.81
CHEMBL1830811 IC50 = 17510.0 nM 4.76
CHEMBL1830824 IC50 = 17780.0 nM 4.75
CHEMBL1830800 IC50 = 17970.0 nM 4.75
CHEMBL1830795 IC50 = 17720.0 nM 4.75
CHEMBL379350 IC50 = 18070.0 nM 4.74
CHEMBL326294 ISATIN IC50 = 18750.0 nM 4.73
CHEMBL1830810 IC50 = 21630.0 nM 4.67
CHEMBL568385 IC50 = 23460.0 nM 4.63
CHEMBL1830801 IC50 = 23840.0 nM 4.62
CHEMBL1830799 IC50 = 27110.0 nM 4.57
CHEMBL1830792 IC50 = 42130.0 nM 4.38
CHEMBL256699 IC50 = 45520.0 nM 4.34
CHEMBL1830834 IC50 = 56120.0 nM 4.25
CHEMBL8706 CLORGILINE IC50 = 61350.0 nM 4.21
CHEMBL1830793 IC50 = 64170.0 nM 4.19