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Assay Detail

CHEMBL1837884

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NF-kappaB activation expressed in TNFalpha-stimulated human HepG2 cells co-transfected with PPRE-Luc plasmid pretreated for 1 hr before TNFalpha challenge measured after 24 hrs by luciferase reporter gene assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type HepG2
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Oleanane-type triterpene saponins from the bark of Aralia elata and their NF-κB inhibition and PPAR activation signal pathway.
Nhiem NX, Lim HY, Kiem PV, Minh CV, Thu VK, Tai BH, Quang TH, Song SB, Kim YH.
Bioorg Med Chem Lett (2011) 21 : 6143 -6147
PubMed 21889336 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 5.49 6.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL421 SULFASALAZINE 4.0 1 6.05
CHEMBL1834950 ELATOSIDE L 1 5.39
CHEMBL1833984 1 5.02

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL421 SULFASALAZINE IC50 = 900.0 nM 6.05
CHEMBL1834950 ELATOSIDE L IC50 = 4100.0 nM 5.39
CHEMBL1833984 IC50 = 9500.0 nM 5.02