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Assay Detail

CHEMBL1838283

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human ERG expressed in CHO-K1 cells after 2 hrs by Cy3b-Dofetilide-based fluorescence polarisation assay
21
Total Activities
21
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Discovery of GSK143, a highly potent, selective and orally efficacious spleen tyrosine kinase inhibitor.
Liddle J, Atkinson FL, Barker MD, Carter PS, Curtis NR, Davis RP, Douault C, Dickson MC, Elwes D, Garton NS, Gray M, Hayhow TG, Hobbs CI, Jones E, Leach S, Leavens K, Lewis HD, McCleary S, Neu M, Patel VK, Preston AG, Ramirez-Molina C, Shipley TJ, Skone PA, Smithers N, Somers DO, Walker AL, Watson RJ, Weingarten GG.
Bioorg Med Chem Lett (2011) 21 : 6188 -6194
PubMed 21903390 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 5.51 6.30
pIC50 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1835057 1 6.30
CHEMBL1835063 1 6.10
CHEMBL1835061 1 6.00
CHEMBL1834758 1 5.80
CHEMBL1835059 1 5.80
CHEMBL515757 1 5.70
CHEMBL1835058 1 5.60
CHEMBL1835060 1 5.60
CHEMBL1834757 1 5.60
CHEMBL1835062 1 5.60
CHEMBL1235110 1 5.50
CHEMBL1835068 1 5.50
CHEMBL1835065 1 5.40
CHEMBL1835067 1 5.40
CHEMBL1835064 1 5.20
CHEMBL1835066 1 5.10
CHEMBL1835069 1 5.10
CHEMBL1835071 1 4.70
CHEMBL1835070 1 4.60
CHEMBL1835072 1 -
CHEMBL1834756 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1835057 IC50 = 501.19 nM 6.30
CHEMBL1835063 IC50 = 794.33 nM 6.10
CHEMBL1835061 IC50 = 1000.0 nM 6.00
CHEMBL1834758 IC50 = 1584.89 nM 5.80
CHEMBL1835059 IC50 = 1584.89 nM 5.80
CHEMBL515757 IC50 = 1995.26 nM 5.70
CHEMBL1835058 IC50 = 2511.89 nM 5.60
CHEMBL1835060 IC50 = 2511.89 nM 5.60
CHEMBL1834757 IC50 = 2511.89 nM 5.60
CHEMBL1835062 IC50 = 2511.89 nM 5.60
CHEMBL1235110 IC50 = 3162.28 nM 5.50
CHEMBL1835068 IC50 = 3162.28 nM 5.50
CHEMBL1835065 IC50 = 3981.07 nM 5.40
CHEMBL1835067 IC50 = 3981.07 nM 5.40
CHEMBL1835064 IC50 = 6309.57 nM 5.20
CHEMBL1835066 IC50 = 7943.28 nM 5.10
CHEMBL1835069 IC50 = 7943.28 nM 5.10
CHEMBL1835071 IC50 = 19952.62 nM 4.70
CHEMBL1835070 IC50 = 25118.86 nM 4.60
CHEMBL1835072 IC50 > 50118.72 nM -
CHEMBL1834756 pIC50 - -