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Assay Detail

CHEMBL1840408

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [33P]ATP from human recombinant FLT3 domain after 20 mins by scintillation counting
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Receptor-type tyrosine-protein kinase FLT3 (CHEMBL1974)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and evaluation of a novel dual FMS-like tyrosine kinase 3/stem cell factor receptor (FLT3/c-KIT) inhibitor for the treatment of acute myelogenous leukemia.
Davies RJ, Pierce AC, Forster C, Grey R, Xu J, Arnost M, Choquette D, Galullo V, Tian SK, Henkel G, Chen G, Heidary DK, Ma J, Stuver-Moody C, Namchuk M.
J Med Chem (2011) 54 : 7184 -7192
PubMed 21970471 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 31 7.82 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1835746 1 9.00
CHEMBL1835870 1 8.70
CHEMBL1835867 1 8.70
CHEMBL1835740 1 8.55
CHEMBL1835871 1 8.30
CHEMBL1835869 1 8.30
CHEMBL1835749 1 8.30
CHEMBL1835747 1 8.30
CHEMBL1833992 1 8.22
CHEMBL1835750 1 8.15
CHEMBL1835748 1 8.10
CHEMBL1835866 1 8.05
CHEMBL1835864 1 8.00
CHEMBL1835736 1 7.96
CHEMBL1835863 1 7.96
CHEMBL1835751 1 7.92
CHEMBL1835865 1 7.92
CHEMBL1835743 1 7.92
CHEMBL1835744 1 7.82
CHEMBL1835741 1 7.82
CHEMBL1835742 1 7.75
CHEMBL1835745 1 7.66
CHEMBL1835662 1 7.66
CHEMBL1835663 1 7.62
CHEMBL1835661 1 7.38
CHEMBL1835739 1 7.28
CHEMBL1835660 1 7.07
CHEMBL1835664 1 6.68
CHEMBL1835738 1 6.03
CHEMBL1835737 1 5.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1835746 Ki = 1.0 nM 9.00
CHEMBL1835870 Ki = 2.0 nM 8.70
CHEMBL1835867 Ki = 2.0 nM 8.70
CHEMBL1835740 Ki = 2.8 nM 8.55
CHEMBL1835871 Ki = 5.0 nM 8.30
CHEMBL1835869 Ki = 5.0 nM 8.30
CHEMBL1835749 Ki = 5.0 nM 8.30
CHEMBL1835747 Ki = 5.0 nM 8.30
CHEMBL1833992 Ki = 6.0 nM 8.22
CHEMBL1835750 Ki = 7.0 nM 8.15
CHEMBL1835748 Ki = 8.0 nM 8.10
CHEMBL1835866 Ki = 9.0 nM 8.05
CHEMBL1835864 Ki = 10.0 nM 8.00
CHEMBL1835736 Ki = 11.0 nM 7.96
CHEMBL1835863 Ki = 11.0 nM 7.96
CHEMBL1835751 Ki = 12.0 nM 7.92
CHEMBL1835865 Ki = 12.0 nM 7.92
CHEMBL1835743 Ki = 12.0 nM 7.92
CHEMBL1835744 Ki = 15.0 nM 7.82
CHEMBL1835741 Ki = 15.0 nM 7.82
CHEMBL1835742 Ki = 18.0 nM 7.75
CHEMBL1835745 Ki = 22.0 nM 7.66
CHEMBL1835662 Ki = 22.0 nM 7.66
CHEMBL1835663 Ki = 24.0 nM 7.62
CHEMBL1835661 Ki = 42.0 nM 7.38
CHEMBL1835739 Ki = 53.0 nM 7.28
CHEMBL1835660 Ki = 85.0 nM 7.07
CHEMBL1835664 Ki = 210.0 nM 6.68
CHEMBL1835738 Ki = 930.0 nM 6.03
CHEMBL1835737 Ki = 2500.0 nM 5.60
CHEMBL1835868 Ki < 1.0 nM -