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Assay Detail

CHEMBL1914005

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of RPTPgamma expressed in Escherichia coli BL21 (DE3) assessed as reduction in dephosphorylation of Z'-Lyte phospho-Tyr 1 peptide preincubated for 20 mins by FRET assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Receptor-type tyrosine-protein phosphatase gamma (CHEMBL4905)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Small molecule receptor protein tyrosine phosphatase γ (RPTPγ) ligands that inhibit phosphatase activity via perturbation of the tryptophan-proline-aspartate (WPD) loop.
Sheriff S, Beno BR, Zhai W, Kostich WA, McDonnell PA, Kish K, Goldfarb V, Gao M, Kiefer SE, Yanchunas J, Huang Y, Shi S, Zhu S, Dzierba C, Bronson J, Macor JE, Appiah KK, Westphal RS, O'Connell J, Gerritz SW.
J Med Chem (2011) 54 : 6548 -6562
PubMed 21882820 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 5.17 6.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1910889 1 6.30
CHEMBL1910968 1 5.89
CHEMBL1403981 1 5.58
CHEMBL1910886 1 5.48
CHEMBL1910887 1 5.47
CHEMBL1910882 1 5.47
CHEMBL1910888 1 5.42
CHEMBL1910966 1 5.40
CHEMBL1910962 1 5.27
CHEMBL1910967 1 5.24
CHEMBL1910963 1 5.24
CHEMBL1910965 1 5.00
CHEMBL1910879 1 4.70
CHEMBL1910964 1 4.11
CHEMBL1910878 1 4.10
CHEMBL1910877 1 4.10
CHEMBL1910969 1 -
CHEMBL1910885 1 -
CHEMBL1910884 1 -
CHEMBL1910883 1 -
CHEMBL1910881 1 -
CHEMBL1910880 1 -
CHEMBL1910876 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1910889 IC50 = 500.0 nM 6.30
CHEMBL1910968 IC50 = 1300.0 nM 5.89
CHEMBL1403981 IC50 = 2600.0 nM 5.58
CHEMBL1910886 IC50 = 3300.0 nM 5.48
CHEMBL1910887 IC50 = 3400.0 nM 5.47
CHEMBL1910882 IC50 = 3400.0 nM 5.47
CHEMBL1910888 IC50 = 3800.0 nM 5.42
CHEMBL1910966 IC50 = 4000.0 nM 5.40
CHEMBL1910962 IC50 = 5400.0 nM 5.27
CHEMBL1910967 IC50 = 5800.0 nM 5.24
CHEMBL1910963 IC50 = 5800.0 nM 5.24
CHEMBL1910965 IC50 = 10000.0 nM 5.00
CHEMBL1910879 IC50 = 20000.0 nM 4.70
CHEMBL1910964 IC50 = 78000.0 nM 4.11
CHEMBL1910878 IC50 = 80000.0 nM 4.10
CHEMBL1910877 IC50 = 80000.0 nM 4.10
CHEMBL1910969 IC50 > 100000.0 nM -
CHEMBL1910885 IC50 > 120000.0 nM -
CHEMBL1910884 IC50 > 120000.0 nM -
CHEMBL1910883 IC50 > 120000.0 nM -
CHEMBL1910881 IC50 > 120000.0 nM -
CHEMBL1910880 IC50 = 110000.0 nM -
CHEMBL1910876 IC50 > 120000.0 nM -