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Assay Detail

CHEMBL1924645

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of FLAP in human peripheral leukocytes assessed as inhibition of calcium ionophore A23187-induced LTB4 production after 1 hr by ELISA in absence of human serum albumin
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Arachidonate 5-lipoxygenase-activating protein (CHEMBL4550)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

5-Lipoxygenase-activating protein (FLAP) inhibitors. Part 4: development of 3-[3-tert-butylsulfanyl-1-[4-(6-ethoxypyridin-3-yl)benzyl]-5-(5-methylpyridin-2-ylmethoxy)-1H-indol-2-yl]-2,2-dimethylpropionic acid (AM803), a potent, oral, once daily FLAP inhibitor.
Stock NS, Bain G, Zunic J, Li Y, Ziff J, Roppe J, Santini A, Darlington J, Prodanovich P, King CD, Baccei C, Lee C, Rong H, Chapman C, Broadhead A, Lorrain D, Correa L, Hutchinson JH, Evans JF, Prasit P.
J Med Chem (2011) 54 : 8013 -8029
PubMed 22059882 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 8.80 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL313489 QUIFLAPON SODIUM -1.0 1 9.00
CHEMBL1922660 FIBOFLAPON 3.0 1 9.00
CHEMBL557527 1 8.96
CHEMBL29097 1 8.24

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL313489 QUIFLAPON SODIUM IC50 = 1.0 nM 9.00
CHEMBL1922660 FIBOFLAPON IC50 = 1.0 nM 9.00
CHEMBL557527 IC50 = 1.1 nM 8.96
CHEMBL29097 IC50 = 5.7 nM 8.24