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Assay Detail

CHEMBL1924801

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human N-terminus peptide (DYKDDDD)-tagged EGFR expressed in baculovirus infected insect cells using [gamma-32P]ATP as substrate after 60 mins by scintillation counting
39
Total Activities
39
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL2363049)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Design and synthesis of novel human epidermal growth factor receptor 2 (HER2)/epidermal growth factor receptor (EGFR) dual inhibitors bearing a pyrrolo[3,2-d]pyrimidine scaffold.
Ishikawa T, Seto M, Banno H, Kawakita Y, Oorui M, Taniguchi T, Ohta Y, Tamura T, Nakayama A, Miki H, Kamiguchi H, Tanaka T, Habuka N, Sogabe S, Yano J, Aertgeerts K, Kamiyama K.
J Med Chem (2011) 54 : 8030 -8050
PubMed 22003817 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 39 7.31 8.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1923009 1 8.24
CHEMBL1923022 1 8.14
CHEMBL1923000 1 8.11
CHEMBL1923003 1 8.04
CHEMBL1923006 1 8.02
CHEMBL1923019 1 8.00
CHEMBL1923010 1 7.82
CHEMBL1923020 1 7.82
CHEMBL1923001 1 7.75
CHEMBL1922999 1 7.70
CHEMBL1923013 1 7.66
CHEMBL1614725 TAK-285 1.0 1 7.64
CHEMBL1923004 1 7.57
CHEMBL1922900 1 7.57
CHEMBL1923015 1 7.51
CHEMBL1923012 1 7.48
CHEMBL1923007 1 7.47
CHEMBL1923021 1 7.46
CHEMBL1921812 1 7.46
CHEMBL1923017 1 7.42
CHEMBL1923016 1 7.40
CHEMBL1923014 1 7.36
CHEMBL1923011 1 7.31
CHEMBL1923005 1 7.30
CHEMBL1922901 1 7.29
CHEMBL1922898 1 7.21
CHEMBL1922905 1 7.17
CHEMBL1923008 1 6.96
CHEMBL1922897 1 6.96
CHEMBL1922904 1 6.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1923009 IC50 = 5.7 nM 8.24
CHEMBL1923022 IC50 = 7.2 nM 8.14
CHEMBL1923000 IC50 = 7.7 nM 8.11
CHEMBL1923003 IC50 = 9.2 nM 8.04
CHEMBL1923006 IC50 = 9.5 nM 8.02
CHEMBL1923019 IC50 = 10.0 nM 8.00
CHEMBL1923010 IC50 = 15.0 nM 7.82
CHEMBL1923020 IC50 = 15.0 nM 7.82
CHEMBL1923001 IC50 = 18.0 nM 7.75
CHEMBL1922999 IC50 = 20.0 nM 7.70
CHEMBL1923013 IC50 = 22.0 nM 7.66
CHEMBL1614725 TAK-285 IC50 = 23.0 nM 7.64
CHEMBL1923004 IC50 = 27.0 nM 7.57
CHEMBL1922900 IC50 = 27.0 nM 7.57
CHEMBL1923015 IC50 = 31.0 nM 7.51
CHEMBL1923012 IC50 = 33.0 nM 7.48
CHEMBL1923007 IC50 = 34.0 nM 7.47
CHEMBL1923021 IC50 = 35.0 nM 7.46
CHEMBL1921812 IC50 = 35.0 nM 7.46
CHEMBL1923017 IC50 = 38.0 nM 7.42
CHEMBL1923016 IC50 = 40.0 nM 7.40
CHEMBL1923014 IC50 = 44.0 nM 7.36
CHEMBL1923011 IC50 = 49.0 nM 7.31
CHEMBL1923005 IC50 = 50.0 nM 7.30
CHEMBL1922901 IC50 = 51.0 nM 7.29
CHEMBL1922898 IC50 = 61.0 nM 7.21
CHEMBL1922905 IC50 = 67.0 nM 7.17
CHEMBL1923008 IC50 = 110.0 nM 6.96
CHEMBL1922897 IC50 = 110.0 nM 6.96
CHEMBL1922904 IC50 = 150.0 nM 6.82
CHEMBL1922899 IC50 = 150.0 nM 6.82
CHEMBL1922902 IC50 = 150.0 nM 6.82
CHEMBL1922906 IC50 = 210.0 nM 6.68
CHEMBL1922998 IC50 = 220.0 nM 6.66
CHEMBL1923018 IC50 = 230.0 nM 6.64
CHEMBL1921813 IC50 = 290.0 nM 6.54
CHEMBL1614726 IC50 = 420.0 nM 6.38
CHEMBL1922903 IC50 = 590.0 nM 6.23
CHEMBL1923002 IC50 = 1900.0 nM 5.72