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Assay Detail

CHEMBL1936154

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of electric eel AChE using acetylcholine as substrate measured every sec for 2 mins by Ellman's method
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Electrophorus electricus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL4078)
Type SINGLE PROTEIN
Organism Electrophorus electricus

Publication

AChE inhibitor: a regio- and stereo-selective 1,3-dipolar cycloaddition for the synthesis of novel substituted 5,6-dimethoxy spiro[5.3']-oxindole-spiro-[6.3″]-2,3-dihydro-1H-inden-1″-one-7-(substituted aryl)-tetrahydro-1H-pyrrolo[1,2-c][1,3]thiazole.
Ashraf Ali M, Ismail R, Choon TS, Kumar RS, Osman H, Arumugam N, Almansour AI, Elumalai K, Singh A.
Bioorg Med Chem Lett (2012) 22 : 508 -511
PubMed 22142546 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.84 6.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1935151 1 6.96
CHEMBL1678 DONEPEZIL HYDROCHLORIDE 4.0 1 6.92
CHEMBL1935146 1 6.47
CHEMBL1935141 1 6.32
CHEMBL1935142 1 5.95
CHEMBL1935152 1 5.94
CHEMBL1935147 1 5.81
CHEMBL1935153 1 5.68
CHEMBL1935154 1 5.67
CHEMBL1935148 1 5.65
CHEMBL1935150 1 5.58
CHEMBL1935155 1 5.50
CHEMBL1935149 1 5.48
CHEMBL1935145 1 5.24
CHEMBL1935143 1 5.21
CHEMBL1935144 1 5.08

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1935151 IC50 = 110.0 nM 6.96
CHEMBL1678 DONEPEZIL HYDROCHLORIDE IC50 = 120.0 nM 6.92
CHEMBL1935146 IC50 = 340.0 nM 6.47
CHEMBL1935141 IC50 = 480.0 nM 6.32
CHEMBL1935142 IC50 = 1120.0 nM 5.95
CHEMBL1935152 IC50 = 1160.0 nM 5.94
CHEMBL1935147 IC50 = 1560.0 nM 5.81
CHEMBL1935153 IC50 = 2100.0 nM 5.68
CHEMBL1935154 IC50 = 2120.0 nM 5.67
CHEMBL1935148 IC50 = 2240.0 nM 5.65
CHEMBL1935150 IC50 = 2600.0 nM 5.58
CHEMBL1935155 IC50 = 3160.0 nM 5.50
CHEMBL1935149 IC50 = 3280.0 nM 5.48
CHEMBL1935145 IC50 = 5800.0 nM 5.24
CHEMBL1935143 IC50 = 6200.0 nM 5.21
CHEMBL1935144 IC50 = 8320.0 nM 5.08