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Assay Detail

CHEMBL1947347

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat recombinant nNOS expressed in baculovirus-infected Sf9 cells assessed as conversion of oxyhemoglobin to methemoglobin by UV spectrophotometer analysis
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nitric oxide synthase 1 (CHEMBL3048)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Novel inhibitors of nitric oxide synthase with antioxidant properties.
Salerno L, Modica MN, Romeo G, Pittalà V, Siracusa MA, Amato ME, Acquaviva R, Di Giacomo C, Sorrenti V.
Eur J Med Chem (2012) 49 : 118 -126
PubMed 22280820 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 30 4.38 4.90

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1529996 1 4.90
CHEMBL1946353 1 4.86
CHEMBL1946359 1 4.65
CHEMBL1946356 1 4.65
CHEMBL1946354 1 4.50
CHEMBL1946357 1 4.49
CHEMBL1946355 1 4.45
CHEMBL1946204 1 4.44
CHEMBL1945914 1 4.40
CHEMBL1945915 1 4.40
CHEMBL1946206 1 4.40
CHEMBL1945916 1 4.35
CHEMBL1946352 1 4.33
CHEMBL1946351 1 4.32
CHEMBL1946207 1 4.20
CHEMBL1946208 1 4.19
CHEMBL1946358 1 4.18
CHEMBL447987 1 4.13
CHEMBL1946203 1 4.12
CHEMBL1945752 1 4.07
CHEMBL1945753 1 4.05
CHEMBL1945917 1 -
CHEMBL1946205 1 -
CHEMBL1548634 1 -
CHEMBL162549 1 -
CHEMBL1945755 1 -
CHEMBL1945754 1 -
CHEMBL1945751 1 -
CHEMBL1945750 1 -
CHEMBL1945749 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1529996 Ki = 12500.0 nM 4.90
CHEMBL1946353 Ki = 13750.0 nM 4.86
CHEMBL1946359 Ki = 22500.0 nM 4.65
CHEMBL1946356 Ki = 22500.0 nM 4.65
CHEMBL1946354 Ki = 31660.0 nM 4.50
CHEMBL1946357 Ki = 32500.0 nM 4.49
CHEMBL1946355 Ki = 35830.0 nM 4.45
CHEMBL1946204 Ki = 36250.0 nM 4.44
CHEMBL1945914 Ki = 40000.0 nM 4.40
CHEMBL1945915 Ki = 40000.0 nM 4.40
CHEMBL1946206 Ki = 40000.0 nM 4.40
CHEMBL1945916 Ki = 45000.0 nM 4.35
CHEMBL1946352 Ki = 46600.0 nM 4.33
CHEMBL1946351 Ki = 47500.0 nM 4.32
CHEMBL1946207 Ki = 63000.0 nM 4.20
CHEMBL1946208 Ki = 65000.0 nM 4.19
CHEMBL1946358 Ki = 66250.0 nM 4.18
CHEMBL447987 Ki = 74000.0 nM 4.13
CHEMBL1946203 Ki = 75000.0 nM 4.12
CHEMBL1945752 Ki = 85000.0 nM 4.07
CHEMBL1945753 Ki = 90000.0 nM 4.05
CHEMBL1945917 Ki = 120000.0 nM -
CHEMBL1946205 Ki = 230000.0 nM -
CHEMBL1548634 Ki = 480000.0 nM -
CHEMBL162549 Ki = 105000.0 nM -
CHEMBL1945755 Ki = 450000.0 nM -
CHEMBL1945754 Ki = 750000.0 nM -
CHEMBL1945751 Ki = 620000.0 nM -
CHEMBL1945750 Ki > 500000.0 nM -
CHEMBL1945749 Ki = 450000.0 nM -