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Assay Detail

CHEMBL1947437

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human Chk2 using STK1 as substrate after 10 mins by HTRF assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase Chk2 (CHEMBL2527)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and evaluation of debromohymenialdisine-derived Chk2 inhibitors.
Saleem RS, Lansdell TA, Tepe JJ.
Bioorg Med Chem (2012) 20 : 1475 -1481
PubMed 22285028 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 7.09 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL332551 1 8.10
CHEMBL1947251 1 7.85
CHEMBL1947252 1 7.85
CHEMBL1947250 1 7.70
CHEMBL1947253 1 7.55
CHEMBL1944822 1 6.81
CHEMBL1944823 1 6.77
CHEMBL255465 DEBROMOHYMENIALDISINE 1 6.74
CHEMBL1944821 1 6.56
CHEMBL1947254 1 6.23
CHEMBL1947255 1 5.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL332551 IC50 = 8.0 nM 8.10
CHEMBL1947251 IC50 = 14.0 nM 7.85
CHEMBL1947252 IC50 = 14.0 nM 7.85
CHEMBL1947250 IC50 = 20.0 nM 7.70
CHEMBL1947253 IC50 = 28.0 nM 7.55
CHEMBL1944822 IC50 = 156.0 nM 6.81
CHEMBL1944823 IC50 = 171.0 nM 6.77
CHEMBL255465 DEBROMOHYMENIALDISINE IC50 = 183.0 nM 6.74
CHEMBL1944821 IC50 = 277.0 nM 6.56
CHEMBL1947254 IC50 = 588.0 nM 6.23
CHEMBL1947255 IC50 = 1526.0 nM 5.82