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Assay Detail

CHEMBL1947453

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Clostridium perfringens ATCC 10543 sialidase Nan1 c-terminal catalytic domain using 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid sodium salt hydrate as substrate by fluorimetric analysis
18
Total Activities
9
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Clostridium perfringens
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Selective and slow-binding inhibition of shikonin derivatives isolated from Lithospermum erythrorhizon on glycosyl hydrolase 33 and 34 sialidases.
Kim JY, Jeong HJ, Park JY, Kim YM, Park SJ, Cho JK, Park KH, Ryu YB, Lee WS.
Bioorg Med Chem (2012) 20 : 1740 -1748
PubMed 22300884 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.56 6.70
Inhibition 9 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1944654 SHIKONOFURAN E 2 6.70
CHEMBL1946217 SHIKONOFURAN C 2 6.22
CHEMBL1946216 SHIKONOFURAN D 2 6.00
CHEMBL267024 DIMETHYLACRYLSHIKONIN 2 5.72
CHEMBL403516 ACETYLSHIKONIN 2 5.60
CHEMBL9416 ISOBUTYLSHIKONIN 2 5.54
CHEMBL397309 BETA-HYDROXYISOVALERYLSHIKONIN 2 5.47
CHEMBL486627 DEOXYSHIKONIN 2 4.56
CHEMBL9470 SHIKONIN 2 4.27

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1944654 SHIKONOFURAN E IC50 = 200.0 nM 6.70
CHEMBL1946217 SHIKONOFURAN C IC50 = 600.0 nM 6.22
CHEMBL1946216 SHIKONOFURAN D IC50 = 1000.0 nM 6.00
CHEMBL267024 DIMETHYLACRYLSHIKONIN IC50 = 1900.0 nM 5.72
CHEMBL403516 ACETYLSHIKONIN IC50 = 2500.0 nM 5.60
CHEMBL9416 ISOBUTYLSHIKONIN IC50 = 2900.0 nM 5.54
CHEMBL397309 BETA-HYDROXYISOVALERYLSHIKONIN IC50 = 3400.0 nM 5.47
CHEMBL486627 DEOXYSHIKONIN IC50 = 27500.0 nM 4.56
CHEMBL9470 SHIKONIN IC50 = 53800.0 nM 4.27
CHEMBL9470 SHIKONIN Inhibition - % -
CHEMBL403516 ACETYLSHIKONIN Inhibition - % -
CHEMBL267024 DIMETHYLACRYLSHIKONIN Inhibition - % -
CHEMBL397309 BETA-HYDROXYISOVALERYLSHIKONIN Inhibition - % -
CHEMBL9416 ISOBUTYLSHIKONIN Inhibition - % -
CHEMBL486627 DEOXYSHIKONIN Inhibition - % -
CHEMBL1946216 SHIKONOFURAN D Inhibition - % -
CHEMBL1944654 SHIKONOFURAN E Inhibition - % -
CHEMBL1946217 SHIKONOFURAN C Inhibition - % -