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Assay Detail

CHEMBL1960409

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at GAL4-tagged human PPARalpha ligand binding domain expressed in HepG2 cells assessed as transactivation after 20 hrs by beta-galactosidase reporter gene assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HepG2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor alpha (CHEMBL239)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis, biological evaluation and molecular investigation of fluorinated peroxisome proliferator-activated receptors α/γ dual agonists.
Fracchiolla G, Laghezza A, Piemontese L, Parente M, Lavecchia A, Pochetti G, Montanari R, Di Giovanni C, Carbonara G, Tortorella P, Novellino E, Loiodice F.
Bioorg Med Chem (2012) 20 : 2141 -2151
PubMed 22341573 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 18 5.30 6.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL192518 1 6.30
CHEMBL1956364 1 6.00
CHEMBL1956363 1 5.85
CHEMBL295416 PIRINIXIC ACID 2.0 1 5.80
CHEMBL1956362 1 5.66
CHEMBL1956366 1 5.60
CHEMBL1956368 1 5.35
CHEMBL364748 1 5.31
CHEMBL1956349 1 5.29
CHEMBL1956350 1 5.27
CHEMBL1956365 1 5.19
CHEMBL1956367 1 5.18
CHEMBL1956360 1 5.01
CHEMBL1956371 1 4.99
CHEMBL1956370 1 4.72
CHEMBL1956358 1 4.68
CHEMBL1956359 1 4.65
CHEMBL1956357 1 4.61

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL192518 EC50 = 500.0 nM 6.30
CHEMBL1956364 EC50 = 1000.0 nM 6.00
CHEMBL1956363 EC50 = 1400.0 nM 5.85
CHEMBL295416 PIRINIXIC ACID EC50 = 1600.0 nM 5.80
CHEMBL1956362 EC50 = 2200.0 nM 5.66
CHEMBL1956366 EC50 = 2500.0 nM 5.60
CHEMBL1956368 EC50 = 4500.0 nM 5.35
CHEMBL364748 EC50 = 4900.0 nM 5.31
CHEMBL1956349 EC50 = 5100.0 nM 5.29
CHEMBL1956350 EC50 = 5400.0 nM 5.27
CHEMBL1956365 EC50 = 6500.0 nM 5.19
CHEMBL1956367 EC50 = 6600.0 nM 5.18
CHEMBL1956360 EC50 = 9800.0 nM 5.01
CHEMBL1956371 EC50 = 10300.0 nM 4.99
CHEMBL1956370 EC50 = 19000.0 nM 4.72
CHEMBL1956358 EC50 = 21000.0 nM 4.68
CHEMBL1956359 EC50 = 22300.0 nM 4.65
CHEMBL1956357 EC50 = 24800.0 nM 4.61