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Assay Detail

CHEMBL1960508

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant carbonic anhydrase 14 preincubated for 15 mins to 72 hrs measured after 6 hrs by stopped flow CO2 hydration method
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Carbonic anhydrase 14 (CHEMBL3510)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

New chemotypes acting as isozyme-selective carbonic anhydrase inhibitors with low affinity for the offtarget cytosolic isoform II.
Carta F, Vullo D, Maresca A, Scozzafava A, Supuran CT.
Bioorg Med Chem Lett (2012) 22 : 2182 -2185
PubMed 22365761 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 6.29 7.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1956579 1 7.80
CHEMBL186422 1 7.44
CHEMBL1956578 1 7.32
CHEMBL1200471 PYRITHIONE ZINC 4.0 1 5.54
CHEMBL251680 COUMAPHOS 2.0 1 5.38
CHEMBL1955873 THIOBENZOIC ACID 1 5.34
CHEMBL508338 THIMEROSAL 3.0 1 5.18
CHEMBL6466 COUMARIN 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1956579 Ki = 16.0 nM 7.80
CHEMBL186422 Ki = 36.0 nM 7.44
CHEMBL1956578 Ki = 48.0 nM 7.32
CHEMBL1200471 PYRITHIONE ZINC Ki = 2910.0 nM 5.54
CHEMBL251680 COUMAPHOS Ki = 4140.0 nM 5.38
CHEMBL1955873 THIOBENZOIC ACID Ki = 4550.0 nM 5.34
CHEMBL508338 THIMEROSAL Ki = 6580.0 nM 5.18
CHEMBL6466 COUMARIN Ki > 200000.0 nM -