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Assay Detail

CHEMBL2014325

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of TNFalpha-induced NFkappaB transcriptional activity in human HepG2 cells pretreated for 1 hr before TNFalpha challenge by luciferase reporter gene analysis
7
Total Activities
4
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HepG2
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Anti-inflammatory and PPAR transactivational effects of secondary metabolites from the roots of Asarum sieboldii.
Quang TH, Ngan NT, Minh CV, Kiem PV, Tai BH, Thao NP, Song SB, Kim YH.
Bioorg Med Chem Lett (2012) 22 : 2527 -2533
PubMed 22381047 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.36 6.05
Inhibition 3 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL421 SULFASALAZINE 4.0 1 6.05
CHEMBL2011545 2 5.19
CHEMBL43469 ASARININ 2 5.15
CHEMBL252915 SESAMIN 2 5.03

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL421 SULFASALAZINE IC50 = 900.0 nM 6.05
CHEMBL2011545 IC50 = 6400.0 nM 5.19
CHEMBL43469 ASARININ IC50 = 7100.0 nM 5.15
CHEMBL252915 SESAMIN IC50 = 9400.0 nM 5.03
CHEMBL2011545 Inhibition - % -
CHEMBL252915 SESAMIN Inhibition - % -
CHEMBL43469 ASARININ Inhibition - % -