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Assay Detail

CHEMBL2014888

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiinflammatory activity in rat RBL2H3 cells assessed as inhibition of DNP-BSA-induced TNF-alpha production preincubated for 15 mins prior DNP-BSA challenge measured after 30 mins by ELISA
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type RBL-2H3
Confidence 1 — Organism
Curated By Autocuration

Target

RBL-2H3 (CHEMBL614632)
Type CELL-LINE
Organism Rattus norvegicus

Publication

Design and synthesis of a vialinin A analog with a potent inhibitory activity of TNF-α production and its transformation into a couple of bioprobes.
Ye YQ, Onose J, Abe N, Koshino H, Takahashi S.
Bioorg Med Chem Lett (2012) 22 : 2385 -2387
PubMed 22410084 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 9.53 10.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2012939 1 10.70
CHEMBL2012938 VIALININ A 1 10.05
CHEMBL269732 TACROLIMUS ANHYDROUS 4.0 1 9.60
CHEMBL2013070 1 9.49
CHEMBL2013071 1 9.26
CHEMBL2013072 1 8.09

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2012939 IC50 = 0.02 nM 10.70
CHEMBL2012938 VIALININ A IC50 = 0.09 nM 10.05
CHEMBL269732 TACROLIMUS ANHYDROUS IC50 = 0.25 nM 9.60
CHEMBL2013070 IC50 = 0.32 nM 9.49
CHEMBL2013071 IC50 = 0.55 nM 9.26
CHEMBL2013072 IC50 = 8.15 nM 8.09