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Assay Detail

CHEMBL2015040

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of CYP1A1 activity in rat liver microsomes using benzo[alpha]pyrene as substrate after 10 mins by fluorescence analysis
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Liver
Subcellular Microsome
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 1A1 (CHEMBL2922)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis and structure elucidation of novel fused 1,2,4-triazine derivatives as potent inhibitors targeting CYP1A1 activity.
El Massry AM, Asal AM, Khattab SN, Haiba NS, Awney HA, Helmy M, Langer V, Amer A.
Bioorg Med Chem (2012) 20 : 2624 -2637
PubMed 22414679 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 7.91 8.27

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2013099 1 8.27
CHEMBL2013100 1 8.26
CHEMBL2013097 1 8.24
CHEMBL2013098 1 8.18
CHEMBL2013101 1 8.11
CHEMBL2013096 1 8.08
CHEMBL2013093 1 8.01
CHEMBL2013094 1 7.98
CHEMBL2013095 1 7.88
CHEMBL2013091 1 7.62
CHEMBL2013102 1 7.21
CHEMBL2013092 1 7.06
CHEMBL2013090 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2013099 IC50 = 5.42 nM 8.27
CHEMBL2013100 IC50 = 5.53 nM 8.26
CHEMBL2013097 IC50 = 5.69 nM 8.24
CHEMBL2013098 IC50 = 6.62 nM 8.18
CHEMBL2013101 IC50 = 7.74 nM 8.11
CHEMBL2013096 IC50 = 8.36 nM 8.08
CHEMBL2013093 IC50 = 9.71 nM 8.01
CHEMBL2013094 IC50 = 10.4 nM 7.98
CHEMBL2013095 IC50 = 13.2 nM 7.88
CHEMBL2013091 IC50 = 24.2 nM 7.62
CHEMBL2013102 IC50 = 61.4 nM 7.21
CHEMBL2013092 IC50 = 86.7 nM 7.06
CHEMBL2013090 IC50 - -