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Assay Detail

CHEMBL2019709

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human PDE4D2 catalytic domain (86 to 413) expressed in Escherichia coli BL21 using [3H]-cAMP/[3H]-cGMP after 15 mins by liquid scintillation counting
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

3',5'-cyclic-AMP phosphodiesterase 4D (CHEMBL288)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Moracin M from Morus alba L. is a natural phosphodiesterase-4 inhibitor.
Chen SK, Zhao P, Shao YX, Li Z, Zhang C, Liu P, He X, Luo HB, Hu X.
Bioorg Med Chem Lett (2012) 22 : 3261 -3264
PubMed 22483586 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 5.49 6.34

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL193240 ROFLUMILAST 4.0 1 6.34
CHEMBL512578 MORACIN M 1 5.54
CHEMBL2018876 MORACIN C 1 4.58

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL193240 ROFLUMILAST IC50 = 460.0 nM 6.34
CHEMBL512578 MORACIN M IC50 = 2900.0 nM 5.54
CHEMBL2018876 MORACIN C IC50 = 26000.0 nM 4.58