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Assay Detail

CHEMBL2019763

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Blockade of human ENaC expressed in HBE cells by short-circuit current assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HBE
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of a novel chemotype of potent human ENaC blockers using a bioisostere approach. Part 2: α-Branched quaternary amines.
Hunt T, Atherton-Watson HC, Collingwood SP, Coote KJ, Czarnecki S, Danahay H, Howsham C, Hunt P, Paisley D, Young A.
Bioorg Med Chem Lett (2012) 22 : 2877 -2879
PubMed 22425452 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 6.64 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL212432 552-02 FREE BASE 2.0 1 8.70
CHEMBL2017996 1 8.40
CHEMBL2017991 1 7.52
CHEMBL2017994 1 7.23
CHEMBL2017995 1 6.77
CHEMBL945 AMILORIDE 4.0 1 6.66
CHEMBL2017990 1 6.54
CHEMBL2017988 1 6.29
CHEMBL2017989 1 6.12
CHEMBL2017993 1 5.62
CHEMBL2017986 1 5.09
CHEMBL2017992 1 4.71
CHEMBL2017985 1 -
CHEMBL2017987 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL212432 552-02 FREE BASE IC50 = 2.0 nM 8.70
CHEMBL2017996 IC50 = 4.0 nM 8.40
CHEMBL2017991 IC50 = 30.0 nM 7.52
CHEMBL2017994 IC50 = 59.0 nM 7.23
CHEMBL2017995 IC50 = 170.0 nM 6.77
CHEMBL945 AMILORIDE IC50 = 220.0 nM 6.66
CHEMBL2017990 IC50 = 290.0 nM 6.54
CHEMBL2017988 IC50 = 510.0 nM 6.29
CHEMBL2017989 IC50 = 750.0 nM 6.12
CHEMBL2017993 IC50 = 2390.0 nM 5.62
CHEMBL2017986 IC50 = 8090.0 nM 5.09
CHEMBL2017992 IC50 = 19510.0 nM 4.71
CHEMBL2017985 IC50 > 10000.0 nM -
CHEMBL2017987 IC50 > 30000.0 nM -