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Assay Detail

CHEMBL2019764

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Blockade of guinea pig ENaC expressed in FRT cells by short-circuit current assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Cavia porcellus
Cell Type FRT
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of a novel chemotype of potent human ENaC blockers using a bioisostere approach. Part 2: α-Branched quaternary amines.
Hunt T, Atherton-Watson HC, Collingwood SP, Coote KJ, Czarnecki S, Danahay H, Howsham C, Hunt P, Paisley D, Young A.
Bioorg Med Chem Lett (2012) 22 : 2877 -2879
PubMed 22425452 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 7.40 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL212432 552-02 FREE BASE 2.0 1 8.40
CHEMBL2017991 1 7.52
CHEMBL945 AMILORIDE 4.0 1 6.27

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL212432 552-02 FREE BASE IC50 = 4.0 nM 8.40
CHEMBL2017991 IC50 = 30.0 nM 7.52
CHEMBL945 AMILORIDE IC50 = 540.0 nM 6.27