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Assay Detail

CHEMBL2021179

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human SGLT2 expressed in CHO cells assessed as [14C]AMG accumulation after 2 hrs by scintillation counting
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium/glucose cotransporter 2 (CHEMBL3884)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of Ipragliflozin (ASP1941): a novel C-glucoside with benzothiophene structure as a potent and selective sodium glucose co-transporter 2 (SGLT2) inhibitor for the treatment of type 2 diabetes mellitus.
Imamura M, Nakanishi K, Suzuki T, Ikegai K, Shiraki R, Ogiyama T, Murakami T, Kurosaki E, Noda A, Kobayashi Y, Yokota M, Koide T, Kosakai K, Ohkura Y, Takeuchi M, Tomiyama H, Ohta M.
Bioorg Med Chem (2012) 20 : 3263 -3279
PubMed 22507206 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 7.01 8.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2018097 1 8.43
CHEMBL2018096 IPRAGLIFLOZIN 4.0 1 8.13
CHEMBL2018090 1 8.01
CHEMBL2017954 1 8.00
CHEMBL2018091 1 7.89
CHEMBL2018095 1 7.85
CHEMBL2018084 1 7.52
CHEMBL2018092 1 7.33
CHEMBL2017956 1 7.16
CHEMBL2018094 1 7.12
CHEMBL2018093 1 7.04
CHEMBL2017955 1 7.00
CHEMBL2018080 1 6.21
CHEMBL2018087 1 6.19
CHEMBL2018085 1 6.17
CHEMBL2018088 1 5.64
CHEMBL2018081 1 5.31
CHEMBL2018086 1 5.20
CHEMBL2018089 1 -
CHEMBL2018083 1 -
CHEMBL2018082 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2018097 IC50 = 3.7 nM 8.43
CHEMBL2018096 IPRAGLIFLOZIN IC50 = 7.4 nM 8.13
CHEMBL2018090 IC50 = 9.8 nM 8.01
CHEMBL2017954 IC50 = 10.0 nM 8.00
CHEMBL2018091 IC50 = 13.0 nM 7.89
CHEMBL2018095 IC50 = 14.0 nM 7.85
CHEMBL2018084 IC50 = 30.0 nM 7.52
CHEMBL2018092 IC50 = 47.0 nM 7.33
CHEMBL2017956 IC50 = 70.0 nM 7.16
CHEMBL2018094 IC50 = 75.0 nM 7.12
CHEMBL2018093 IC50 = 92.0 nM 7.04
CHEMBL2017955 IC50 = 100.0 nM 7.00
CHEMBL2018080 IC50 = 610.0 nM 6.21
CHEMBL2018087 IC50 = 650.0 nM 6.19
CHEMBL2018085 IC50 = 675.0 nM 6.17
CHEMBL2018088 IC50 = 2300.0 nM 5.64
CHEMBL2018081 IC50 = 4900.0 nM 5.31
CHEMBL2018086 IC50 = 6300.0 nM 5.20
CHEMBL2018089 IC50 > 100000.0 nM -
CHEMBL2018083 IC50 > 100000.0 nM -
CHEMBL2018082 IC50 > 100000.0 nM -