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Assay Detail

CHEMBL2024697

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [35S]TBPS from GABAA receptor picrotoxin binding site in rat brain membranes
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Subcellular Brain membranes
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Neurosteroid analogues. 17. Inverted binding orientations of androsterone enantiomers at the steroid potentiation site on γ-aminobutyric acid type A receptors.
Krishnan K, Manion BD, Taylor A, Bracamontes J, Steinbach JH, Reichert DE, Evers AS, Zorumski CF, Mennerick S, Covey DF.
J Med Chem (2012) 55 : 1334 -1345
PubMed 22191644 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 6.60 7.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2021957 1 7.82
CHEMBL3138531 1 7.28
CHEMBL207538 BREXANOLONE 4.0 1 7.13
CHEMBL429756 1 6.98
CHEMBL3138532 1 6.78
CHEMBL2021955 1 6.54
CHEMBL3138446 1 6.51
CHEMBL87285 ANDROSTERONE 1 6.39
CHEMBL3138444 1 6.33
CHEMBL3138534 1 5.69
CHEMBL2021961 1 5.17
CHEMBL3138533 1 -
CHEMBL2021959 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2021957 IC50 = 15.0 nM 7.82
CHEMBL3138531 IC50 = 52.0 nM 7.28
CHEMBL207538 BREXANOLONE IC50 = 74.0 nM 7.13
CHEMBL429756 IC50 = 105.0 nM 6.98
CHEMBL3138532 IC50 = 168.0 nM 6.78
CHEMBL2021955 IC50 = 285.0 nM 6.54
CHEMBL3138446 IC50 = 311.0 nM 6.51
CHEMBL87285 ANDROSTERONE IC50 = 410.0 nM 6.39
CHEMBL3138444 IC50 = 467.0 nM 6.33
CHEMBL3138534 IC50 = 2050.0 nM 5.69
CHEMBL2021961 IC50 = 6750.0 nM 5.17
CHEMBL3138533 IC50 > 10000.0 nM -
CHEMBL2021959 IC50 > 10000.0 nM -