Assay Detail
Binding
CHEMBL2024713
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of bovine PDE5 after 24 hrs by time-resolved fluorescence resonance energy transfer assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Bos taurus |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
cGMP-specific 3',5'-cyclic phosphodiesterase (CHEMBL3478) ↗| Type | SINGLE PROTEIN |
| Organism | Bos taurus |
Publication
Drug-to-genome-to-drug, step 2: reversing selectivity in a series of antiplasmodial compounds.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 15 | 7.75 | 9.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2022215 | — | — | 1 | 9.00 |
| CHEMBL2022213 | — | — | 1 | 9.00 |
| CHEMBL2022212 | — | — | 1 | 8.70 |
| CHEMBL2022214 | — | — | 1 | 8.52 |
| CHEMBL779 | TADALAFIL | 4.0 | 1 | 8.30 |
| CHEMBL32255 | — | — | 1 | 8.30 |
| CHEMBL2022209 | — | — | 1 | 8.15 |
| CHEMBL2022219 | — | — | 1 | 7.55 |
| CHEMBL2022222 | — | — | 1 | 7.25 |
| CHEMBL284745 | — | — | 1 | 5.28 |
| CHEMBL2024109 | — | — | 1 | 5.25 |
| CHEMBL2024110 | — | — | 1 | - |
| CHEMBL2024108 | — | — | 1 | - |
| CHEMBL2024107 | — | — | 1 | - |
| CHEMBL2022223 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2022215 | — | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL2022213 | — | IC50 | = | 1.0 | nM | 9.00 |
| CHEMBL2022212 | — | IC50 | = | 2.0 | nM | 8.70 |
| CHEMBL2022214 | — | IC50 | = | 3.0 | nM | 8.52 |
| CHEMBL779 | TADALAFIL | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL32255 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL2022209 | — | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL2022219 | — | IC50 | = | 28.0 | nM | 7.55 |
| CHEMBL2022222 | — | IC50 | = | 56.0 | nM | 7.25 |
| CHEMBL284745 | — | IC50 | = | 5200.0 | nM | 5.28 |
| CHEMBL2024109 | — | IC50 | = | 5600.0 | nM | 5.25 |
| CHEMBL2024110 | — | IC50 | > | 10000.0 | nM | - |
| CHEMBL2024108 | — | IC50 | > | 10000.0 | nM | - |
| CHEMBL2024107 | — | IC50 | > | 10000.0 | nM | - |
| CHEMBL2022223 | — | IC50 | > | 1000.0 | nM | - |