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Assay Detail

CHEMBL2024744

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal hexa-histidine tagged human cloned Eg5 (1 to 368 amino acids) expressed in Escherichia coli BL21 (DE3) assessed as reduction of MT-stimulated ATPase activity by pyruvate kinase/lactate dehydrogenase-linked assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Kinesin-like protein KIF11 (CHEMBL4581)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Triphenylbutanamines: kinesin spindle protein inhibitors with in vivo antitumor activity.
Wang F, Good JA, Rath O, Kaan HY, Sutcliffe OB, Mackay SP, Kozielski F.
J Med Chem (2012) 55 : 1511 -1525
PubMed 22248262 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 19 7.65 8.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL228814 ISPINESIB 2.0 1 8.60
CHEMBL2023001 1 8.47
CHEMBL261613 1 8.38
CHEMBL2022994 1 8.29
CHEMBL392369 1 8.04
CHEMBL2022999 1 8.01
CHEMBL2023002 1 8.01
CHEMBL2022996 1 7.99
CHEMBL2022998 1 7.97
CHEMBL2022997 1 7.97
CHEMBL2022995 1 7.89
CHEMBL429597 1 7.87
CHEMBL2023000 1 7.71
CHEMBL392695 S-TRITYL-L-CYSTEINE 1 6.84
CHEMBL408072 1 6.82
CHEMBL2022591 1 6.69
CHEMBL2022590 1 6.67
CHEMBL2022588 1 6.63
CHEMBL2022589 1 6.42

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL228814 ISPINESIB Ki = 2.5 nM 8.60
CHEMBL2023001 Ki = 3.4 nM 8.47
CHEMBL261613 Ki = 4.2 nM 8.38
CHEMBL2022994 Ki = 5.1 nM 8.29
CHEMBL392369 Ki = 9.1 nM 8.04
CHEMBL2022999 Ki = 9.8 nM 8.01
CHEMBL2023002 Ki = 9.7 nM 8.01
CHEMBL2022996 Ki = 10.2 nM 7.99
CHEMBL2022998 Ki = 10.7 nM 7.97
CHEMBL2022997 Ki = 10.8 nM 7.97
CHEMBL2022995 Ki = 12.8 nM 7.89
CHEMBL429597 Ki = 13.6 nM 7.87
CHEMBL2023000 Ki = 19.3 nM 7.71
CHEMBL392695 S-TRITYL-L-CYSTEINE Ki = 143.8 nM 6.84
CHEMBL408072 Ki = 152.3 nM 6.82
CHEMBL2022591 Ki = 203.0 nM 6.69
CHEMBL2022590 Ki = 211.3 nM 6.67
CHEMBL2022588 Ki = 235.4 nM 6.63
CHEMBL2022589 Ki = 384.4 nM 6.42