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Assay Detail

CHEMBL2025957

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H] epibatidine from rat alpha4beta4 nAChR by beta scintillation counting
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta4 (CHEMBL1907595)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Chemistry and behavioral studies identify chiral cyclopropanes as selective α4β2-nicotinic acetylcholine receptor partial agonists exhibiting an antidepressant profile.
Zhang H, Tückmantel W, Eaton JB, Yuen PW, Yu LF, Bajjuri KM, Fedolak A, Wang D, Ghavami A, Caldarone B, Paterson NE, Lowe DA, Brunner D, Lukas RJ, Kozikowski AP.
J Med Chem (2012) 55 : 717 -724
PubMed 22171543 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 6.92 7.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3 NICOTINE 4.0 1 7.64
CHEMBL2024088 1 7.54
CHEMBL2024094 1 7.30
CHEMBL2024087 1 7.08
CHEMBL2024095 1 7.01
CHEMBL2024092 1 6.79
CHEMBL2024091 1 6.63
CHEMBL2024090 1 6.55
CHEMBL2024089 1 6.36
CHEMBL2024093 1 6.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3 NICOTINE Ki = 23.0 nM 7.64
CHEMBL2024088 Ki = 29.0 nM 7.54
CHEMBL2024094 Ki = 50.2 nM 7.30
CHEMBL2024087 Ki = 82.6 nM 7.08
CHEMBL2024095 Ki = 96.5 nM 7.01
CHEMBL2024092 Ki = 162.0 nM 6.79
CHEMBL2024091 Ki = 234.0 nM 6.63
CHEMBL2024090 Ki = 281.0 nM 6.55
CHEMBL2024089 Ki = 441.0 nM 6.36
CHEMBL2024093 Ki = 551.0 nM 6.26