Assay Detail
Binding
CHEMBL2026722
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Estrogenic activity at estrogen receptor in human MCF7:D5L cells co-expressing ERE assessed as induction of luciferase gene expression after 16 hrs by microplate reader
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Ester and carbamate ester derivatives of Biochanin A: synthesis and in vitro evaluation of estrogenic and antiproliferative activities.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 7 | 7.26 | 10.71 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL135 | ESTRADIOL | 4.0 | 1 | 10.71 |
| CHEMBL2022004 | — | — | 1 | 6.85 |
| CHEMBL2022003 | — | — | 1 | 6.85 |
| CHEMBL2022005 | — | — | 1 | 6.75 |
| CHEMBL2022006 | — | — | 1 | 6.66 |
| CHEMBL131921 | BIOCHANIN | — | 1 | 6.52 |
| CHEMBL2022007 | — | — | 1 | 6.50 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL135 | ESTRADIOL | EC50 | = | 0.0196 | nM | 10.71 |
| CHEMBL2022004 | — | EC50 | = | 140.0 | nM | 6.85 |
| CHEMBL2022003 | — | EC50 | = | 140.0 | nM | 6.85 |
| CHEMBL2022005 | — | EC50 | = | 180.0 | nM | 6.75 |
| CHEMBL2022006 | — | EC50 | = | 220.0 | nM | 6.66 |
| CHEMBL131921 | BIOCHANIN | EC50 | = | 300.0 | nM | 6.52 |
| CHEMBL2022007 | — | EC50 | = | 320.0 | nM | 6.50 |