Assay Detail
Functional
CHEMBL2027596
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at human PPARalpha expressed in MCF7 cells co-transfected CPTI DR1-type RE after 16 hrs by luciferase reporter gene assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | MCF7 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Peroxisome proliferator-activated receptor alpha (CHEMBL239) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Discovery of Potent Dual PPARα Agonists/CB1 Ligands.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 6 | 6.29 | 6.82 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL280065 | OLEOYLETHANOLAMIDE | — | 1 | 6.82 |
| CHEMBL295416 | PIRINIXIC ACID | 2.0 | 1 | 6.19 |
| CHEMBL2022404 | — | — | 1 | 6.13 |
| CHEMBL2022403 | — | — | 1 | 6.00 |
| CHEMBL2022405 | — | — | 1 | - |
| CHEMBL2022406 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL280065 | OLEOYLETHANOLAMIDE | EC50 | = | 152.0 | nM | 6.82 |
| CHEMBL295416 | PIRINIXIC ACID | EC50 | = | 650.0 | nM | 6.19 |
| CHEMBL2022404 | — | EC50 | = | 743.0 | nM | 6.13 |
| CHEMBL2022403 | — | EC50 | = | 989.0 | nM | 6.00 |
| CHEMBL2022405 | — | EC50 | - | — | - | |
| CHEMBL2022406 | — | EC50 | - | — | - |