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Assay Detail

CHEMBL2033708

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal hexaHis-tagged Toxoplasma gondii CDPK1 expressed in Escherichia coli BL21* by nonradioactive Kinaseglo luciferase assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Toxoplasma gondii
Tissue Plasma
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Multiple determinants for selective inhibition of apicomplexan calcium-dependent protein kinase CDPK1.
Larson ET, Ojo KK, Murphy RC, Johnson SM, Zhang Z, Kim JE, Leibly DJ, Fox AM, Reid MC, Dale EJ, Perera BG, Kim J, Hewitt SN, Hol WG, Verlinde CL, Fan E, Van Voorhis WC, Maly DJ, Merritt EA.
J Med Chem (2012) 55 : 2803 -2810
PubMed 22369268 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 16 8.24 8.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2030558 1 8.96
CHEMBL2030552 1 8.89
CHEMBL2030554 1 8.89
CHEMBL1241489 1 8.60
CHEMBL2030553 1 8.60
CHEMBL1785021 1 8.60
CHEMBL1785018 1 8.60
CHEMBL1785020 1 8.52
CHEMBL2030556 1 8.34
CHEMBL2030561 1 8.12
CHEMBL2030555 1 8.05
CHEMBL2030557 1 8.05
CHEMBL1242754 1 7.92
CHEMBL2030560 1 6.92
CHEMBL685 MEBENDAZOLE 4.0 1 6.47
CHEMBL2030559 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2030558 Ki = 1.1 nM 8.96
CHEMBL2030552 Ki = 1.3 nM 8.89
CHEMBL2030554 Ki = 1.3 nM 8.89
CHEMBL1241489 Ki = 2.5 nM 8.60
CHEMBL2030553 Ki = 2.5 nM 8.60
CHEMBL1785021 Ki = 2.5 nM 8.60
CHEMBL1785018 Ki = 2.5 nM 8.60
CHEMBL1785020 Ki = 3.0 nM 8.52
CHEMBL2030556 Ki = 4.6 nM 8.34
CHEMBL2030561 Ki = 7.5 nM 8.12
CHEMBL2030555 Ki = 9.0 nM 8.05
CHEMBL2030557 Ki = 9.0 nM 8.05
CHEMBL1242754 Ki = 12.0 nM 7.92
CHEMBL2030560 Ki = 120.0 nM 6.92
CHEMBL685 MEBENDAZOLE Ki = 340.0 nM 6.47
CHEMBL2030559 Ki > 2500.0 nM -