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Assay Detail

CHEMBL2045142

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Smo in mouse C3H10T1/2 cells using human recombinant SHH assessed as effect on SMO/SHH transient transcriptional activation after 20 hrs by Gli-luciferase reporter assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type C3H 10T1/2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein smoothened (CHEMBL6080)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Discovery of PF-04449913, a Potent and Orally Bioavailable Inhibitor of Smoothened.
Munchhof MJ, Li Q, Shavnya A, Borzillo GV, Boyden TL, Jones CS, LaGreca SD, Martinez-Alsina L, Patel N, Pelletier K, Reiter LA, Robbins MD, Tkalcevic GT.
ACS Med Chem Lett (2012) 3 : 106 -111
PubMed 24900436 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 7.58 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2043430 1 8.30
CHEMBL2043435 1 8.30
CHEMBL2043437 GLASDEGIB 4.0 1 8.30
CHEMBL473417 VISMODEGIB 4.0 1 8.30
CHEMBL2043436 1 7.70
CHEMBL2043434 1 7.46
CHEMBL2043432 1 7.37
CHEMBL2043433 1 7.35
CHEMBL2043438 1 7.14
CHEMBL2043431 1 7.06
CHEMBL2043429 1 6.99
CHEMBL2043439 1 6.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2043430 IC50 = 5.0 nM 8.30
CHEMBL2043435 IC50 = 5.0 nM 8.30
CHEMBL2043437 GLASDEGIB IC50 = 5.0 nM 8.30
CHEMBL473417 VISMODEGIB IC50 = 5.0 nM 8.30
CHEMBL2043436 IC50 = 20.0 nM 7.70
CHEMBL2043434 IC50 = 35.0 nM 7.46
CHEMBL2043432 IC50 = 43.0 nM 7.37
CHEMBL2043433 IC50 = 45.0 nM 7.35
CHEMBL2043438 IC50 = 72.0 nM 7.14
CHEMBL2043431 IC50 = 88.0 nM 7.06
CHEMBL2043429 IC50 = 103.0 nM 6.99
CHEMBL2043439 IC50 = 191.0 nM 6.72