Assay Detail
Binding
CHEMBL2060259
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of eIF4A1-mediated protein synthesis in human BJAB cells assessed as inhibition of [35S]Met incorporation after 1 hr by scintillation counting
2
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | BJAB |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Eukaryotic initiation factor 4A-I (CHEMBL2052028) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Synthesis of rocaglamide hydroxamates and related compounds as eukaryotic translation inhibitors: synthetic and biological studies.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 1 | 7.70 | 7.70 |
| Inhibition | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2058177 | — | — | 1 | 7.70 |
| CHEMBL555196 | SILVESTROL | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2058177 | — | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL555196 | SILVESTROL | Inhibition | - | % | - |