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Assay Detail

CHEMBL2060673

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of gamma secretase-mediated amyloid beta42 production in human H4 cells expressing human APP swedish mutant
21
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type H4
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Gamma-secretase (CHEMBL2094135)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Application of the bicyclo[1.1.1]pentane motif as a nonclassical phenyl ring bioisostere in the design of a potent and orally active γ-secretase inhibitor.
Stepan AF, Subramanyam C, Efremov IV, Dutra JK, O'Sullivan TJ, DiRico KJ, McDonald WS, Won A, Dorff PH, Nolan CE, Becker SL, Pustilnik LR, Riddell DR, Kauffman GW, Kormos BL, Zhang L, Lu Y, Capetta SH, Green ME, Karki K, Sibley E, Atchison KP, Hallgren AJ, Oborski CE, Robshaw AE, Sneed B, O'Donnell CJ.
J Med Chem (2012) 55 : 3414 -3424
PubMed 22420884 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 8.19 9.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2059813 1 9.75
CHEMBL1090771 AVAGACESTAT 2.0 1 9.65
CHEMBL2059021 1 9.00
CHEMBL2059814 1 8.98
CHEMBL2059815 1 8.75
CHEMBL2059016 2 8.56
CHEMBL2059011 1 8.32
CHEMBL2059819 1 8.16
CHEMBL2059014 1 8.13
CHEMBL2059015 1 8.02
CHEMBL2059818 1 7.96
CHEMBL2059018 1 7.92
CHEMBL2059020 1 7.91
CHEMBL2059820 1 7.83
CHEMBL2059012 1 7.79
CHEMBL2059816 1 7.65
CHEMBL2059017 1 7.61
CHEMBL2059019 1 7.34
CHEMBL2059013 1 7.23
CHEMBL2059817 1 7.06

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2059813 IC50 = 0.178 nM 9.75
CHEMBL1090771 AVAGACESTAT IC50 = 0.225 nM 9.65
CHEMBL2059021 IC50 = 0.99 nM 9.00
CHEMBL2059814 IC50 = 1.04 nM 8.98
CHEMBL2059815 IC50 = 1.77 nM 8.75
CHEMBL2059016 IC50 = 2.75 nM 8.56
CHEMBL2059016 IC50 = 4.11 nM 8.39
CHEMBL2059011 IC50 = 4.77 nM 8.32
CHEMBL2059819 IC50 = 6.92 nM 8.16
CHEMBL2059014 IC50 = 7.47 nM 8.13
CHEMBL2059015 IC50 = 9.6 nM 8.02
CHEMBL2059818 IC50 = 11.1 nM 7.96
CHEMBL2059018 IC50 = 12.1 nM 7.92
CHEMBL2059020 IC50 = 12.3 nM 7.91
CHEMBL2059820 IC50 = 14.7 nM 7.83
CHEMBL2059012 IC50 = 16.4 nM 7.79
CHEMBL2059816 IC50 = 22.5 nM 7.65
CHEMBL2059017 IC50 = 24.4 nM 7.61
CHEMBL2059019 IC50 = 45.5 nM 7.34
CHEMBL2059013 IC50 = 58.7 nM 7.23
CHEMBL2059817 IC50 = 87.1 nM 7.06