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Assay Detail

CHEMBL2061569

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-1alpha,25(OH)2D3 from recombinant rat VDR after overnight incubation by scintillation counting
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Vitamin D3 receptor (CHEMBL3150)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

A 20S combined with a 22R configuration markedly increases both in vivo and in vitro biological activity of 1α,25-dihydroxy-22-methyl-2-methylene-19-norvitamin D3.
Flores A, Sicinski RR, Grzywacz P, Thoden JB, Plum LA, Clagett-Dame M, DeLuca HF.
J Med Chem (2012) 55 : 4352 -4366
PubMed 22490010 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 9.88 10.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2059272 1 10.70
CHEMBL2059269 1 10.40
CHEMBL2059271 1 10.22
CHEMBL846 CALCITRIOL 4.0 1 10.00
CHEMBL605525 DP001 2.0 1 10.00
CHEMBL2059268 1 10.00
CHEMBL2059267 1 9.70
CHEMBL2059270 1 8.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2059272 Ki = 0.02 nM 10.70
CHEMBL2059269 Ki = 0.04 nM 10.40
CHEMBL2059271 Ki = 0.06 nM 10.22
CHEMBL846 CALCITRIOL Ki = 0.1 nM 10.00
CHEMBL605525 DP001 Ki = 0.1 nM 10.00
CHEMBL2059268 Ki = 0.1 nM 10.00
CHEMBL2059267 Ki = 0.2 nM 9.70
CHEMBL2059270 Ki = 10.0 nM 8.00