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Assay Detail

CHEMBL2061902

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of G6PD in HEK293T cells assessed as accumulation of 6-phosphogluconate after 5 hrs by LC-MS/MS analysis using 6-aminonicotinamide
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK-293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glucose-6-phosphate 1-dehydrogenase (CHEMBL5347)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel steroid inhibitors of glucose 6-phosphate dehydrogenase.
Hamilton NM, Dawson M, Fairweather EE, Hamilton NS, Hitchin JR, James DI, Jones SD, Jordan AM, Lyons AJ, Small HF, Thomson GJ, Waddell ID, Ogilvie DJ.
J Med Chem (2012) 55 : 4431 -4445
PubMed 22506561 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 4.90 5.32

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2057307 1 5.32
CHEMBL2057296 1 5.11
CHEMBL2057310 1 5.04
CHEMBL2057293 1 5.02
CHEMBL2057971 1 4.91
CHEMBL2057640 1 4.85
CHEMBL2057300 1 4.76
CHEMBL90593 PRASTERONE 4.0 1 4.60
CHEMBL2057318 1 4.45

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2057307 IC50 = 4800.0 nM 5.32
CHEMBL2057296 IC50 = 7800.0 nM 5.11
CHEMBL2057310 IC50 = 9200.0 nM 5.04
CHEMBL2057293 IC50 = 9500.0 nM 5.02
CHEMBL2057971 IC50 = 12300.0 nM 4.91
CHEMBL2057640 IC50 = 14100.0 nM 4.85
CHEMBL2057300 IC50 = 17400.0 nM 4.76
CHEMBL90593 PRASTERONE IC50 = 24900.0 nM 4.60
CHEMBL2057318 IC50 = 35200.0 nM 4.45