Assay Detail
Binding
CHEMBL2065334
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Displacement of [3H]WIN-35,428 from human DAT expressed in mouse N2A cells after 15 mins by liquid scintillation counting
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | N2a |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Sodium-dependent dopamine transporter (CHEMBL238) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Evolution of a Compact Photoprobe for the Dopamine Transporter Based on (±)-threo-Methylphenidate.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 8 | 8.01 | 8.74 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1179011 | — | — | 1 | 8.74 |
| CHEMBL2062931 | — | — | 1 | 8.40 |
| CHEMBL1641691 | — | — | 1 | 8.35 |
| CHEMBL1253709 | — | — | 1 | 8.20 |
| CHEMBL2062932 | — | — | 1 | 8.04 |
| CHEMBL827 | DEXMETHYLPHENIDATE | 4.0 | 1 | 7.80 |
| CHEMBL2062933 | — | — | 1 | 7.46 |
| CHEMBL1254439 | — | — | 1 | 7.11 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1179011 | — | Ki | = | 1.8 | nM | 8.74 |
| CHEMBL2062931 | — | Ki | = | 4.0 | nM | 8.40 |
| CHEMBL1641691 | — | Ki | = | 4.5 | nM | 8.35 |
| CHEMBL1253709 | — | Ki | = | 6.3 | nM | 8.20 |
| CHEMBL2062932 | — | Ki | = | 9.1 | nM | 8.04 |
| CHEMBL827 | DEXMETHYLPHENIDATE | Ki | = | 16.0 | nM | 7.80 |
| CHEMBL2062933 | — | Ki | = | 35.0 | nM | 7.46 |
| CHEMBL1254439 | — | Ki | = | 77.0 | nM | 7.11 |