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Assay Detail

CHEMBL2078545

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
TP_TRANSPORTER: inhibition of JC-1 efflux in NIH-3T3-G185 cells
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Cell Type NIH-3T3-G185
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

ATP-dependent translocase ABCB1 (CHEMBL4302)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Active transport of fluorescent P-glycoprotein substrates: evaluation as markers and interaction with inhibitors.
Wang EJ, Casciano CN, Clement RP, Johnson WW.
Biochem Biophys Res Commun (2001) 289 : 580 -585
PubMed 11716514 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 4.88 5.37

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL160 CYCLOSPORINE 4.0 1 5.37
CHEMBL772 RESERPINE 4.0 1 5.21
CHEMBL1484 NICARDIPINE 4.0 1 5.15
CHEMBL6966 VERAPAMIL 4.0 1 4.38
CHEMBL1741 CLARITHROMYCIN 4.0 1 4.30
CHEMBL50588 EMETINE 4.0 1 -
CHEMBL170 QUININE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL160 CYCLOSPORINE IC50 = 4300.0 nM 5.37
CHEMBL772 RESERPINE IC50 = 6100.0 nM 5.21
CHEMBL1484 NICARDIPINE IC50 = 7100.0 nM 5.15
CHEMBL6966 VERAPAMIL IC50 = 42000.0 nM 4.38
CHEMBL1741 CLARITHROMYCIN IC50 = 50200.0 nM 4.30
CHEMBL50588 EMETINE IC50 = 115600.0 nM -
CHEMBL170 QUININE IC50 = 159300.0 nM -