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Assay Detail

CHEMBL2149157

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mPGES1 in IL-1beta treated human A549 cell microsomal membrane assessed as inhibition of PGE2 formation incubated for 15 mins before addition of PGH2 by RP-HPLC method
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type A549
Subcellular Microsome
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin E synthase (CHEMBL5658)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Lignan derivatives from Krameria lappacea roots inhibit acute inflammation in vivo and pro-inflammatory mediators in vitro.
Baumgartner L, Sosa S, Atanasov AG, Bodensieck A, Fakhrudin N, Bauer J, Favero GD, Ponti C, Heiss EH, Schwaiger S, Ladurner A, Widowitz U, Loggia RD, Rollinger JM, Werz O, Bauer R, Dirsch VM, Tubaro A, Stuppner H.
J Nat Prod (2011) 74 : 1779 -1786
PubMed 21800856 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 4.92 5.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL29097 1 5.68
CHEMBL2147422 1 5.28
CHEMBL2147420 1 5.13
CHEMBL463574 RATANIAPHENOL II 1 4.96
CHEMBL2147424 RATANIAPHENOL I 1 4.89
CHEMBL2147423 RATANIAPHENOL III 1 4.87
CHEMBL2147421 1 4.71
CHEMBL2147418 1 4.40
CHEMBL2147419 1 4.38
CHEMBL2147415 1 -
CHEMBL2147416 1 -
CHEMBL2147417 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL29097 IC50 = 2100.0 nM 5.68
CHEMBL2147422 IC50 = 5300.0 nM 5.28
CHEMBL2147420 IC50 = 7400.0 nM 5.13
CHEMBL463574 RATANIAPHENOL II IC50 = 11000.0 nM 4.96
CHEMBL2147424 RATANIAPHENOL I IC50 = 13000.0 nM 4.89
CHEMBL2147423 RATANIAPHENOL III IC50 = 13500.0 nM 4.87
CHEMBL2147421 IC50 = 19500.0 nM 4.71
CHEMBL2147418 IC50 = 39500.0 nM 4.40
CHEMBL2147419 IC50 = 42000.0 nM 4.38
CHEMBL2147415 IC50 > 50000.0 nM -
CHEMBL2147416 IC50 > 50000.0 nM -
CHEMBL2147417 IC50 > 50000.0 nM -