Assay Detail
Binding
CHEMBL2149729
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of SARS-CoV PLpro deubiququitination expressed in Escherichia coli BL21 (DE3) using Arg-Leu-Arg-Gly-Gly-AMC as substrate by fluorescence analysis
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Severe acute respiratory syndrome-related coronavirus |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Replicase polyprotein 1a (CHEMBL3927) ↗| Type | SINGLE PROTEIN |
| Organism | Severe acute respiratory syndrome-related coronavirus |
Publication
Tanshinones as selective and slow-binding inhibitors for SARS-CoV cysteine proteases.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 4.93 | 6.16 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL363535 | TANSHINONE I | — | 1 | 6.16 |
| CHEMBL227075 | DIHYDROTANSHINONE I | — | 1 | 5.92 |
| CHEMBL215254 | TANSHINONE IIB | — | 1 | 4.28 |
| CHEMBL45830 | MILTIRONE | — | 1 | 4.22 |
| CHEMBL187460 | CRYPTOTANSHINONE | — | 1 | 4.06 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL363535 | TANSHINONE I | IC50 | = | 700.0 | nM | 6.16 |
| CHEMBL227075 | DIHYDROTANSHINONE I | IC50 | = | 1200.0 | nM | 5.92 |
| CHEMBL215254 | TANSHINONE IIB | IC50 | = | 52000.0 | nM | 4.28 |
| CHEMBL45830 | MILTIRONE | IC50 | = | 60200.0 | nM | 4.22 |
| CHEMBL187460 | CRYPTOTANSHINONE | IC50 | = | 87600.0 | nM | 4.06 |