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Assay Detail

CHEMBL2154209

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Transactivation of PPARalpha expressed in HEK293A cells co-expressing GAL4 after 16 to 18 hrs by luciferase reporter gene assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293-A
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor alpha (CHEMBL239)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Fibrate-derived N-(methylsulfonyl)amides with antagonistic properties on PPARα.
Ammazzalorso A, D'Angelo A, Giancristofaro A, De Filippis B, Di Matteo M, Fantacuzzi M, Giampietro L, Linciano P, Maccallini C, Amoroso R.
Eur J Med Chem (2012) 58 : 317 -322
PubMed 23137448 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 5 5.38 6.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL21241 GW7647 1 6.70
CHEMBL2153833 1 5.80
CHEMBL2153843 1 5.15
CHEMBL2153840 1 4.97
CHEMBL683 CLOFIBRIC ACID 2.0 1 4.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL21241 GW7647 EC50 = 200.0 nM 6.70
CHEMBL2153833 EC50 = 1590.0 nM 5.80
CHEMBL2153843 EC50 = 7100.0 nM 5.15
CHEMBL2153840 EC50 = 10700.0 nM 4.97
CHEMBL683 CLOFIBRIC ACID EC50 = 55000.0 nM 4.26