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Assay Detail

CHEMBL2160364

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of JAK1 in human TF1 cells assessed as inhibition of IL6-induced STAT3 phosphorylation incubated for 20 mins prior to IL6-induction measured after 30 to 45 mins
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type TF-1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK1 (CHEMBL2835)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and optimization of C-2 methyl imidazopyrrolopyridines as potent and orally bioavailable JAK1 inhibitors with selectivity over JAK2.
Zak M, Mendonca R, Balazs M, Barrett K, Bergeron P, Blair WS, Chang C, Deshmukh G, Devoss J, Dragovich PS, Eigenbrot C, Ghilardi N, Gibbons P, Gradl S, Hamman C, Hanan EJ, Harstad E, Hewitt PR, Hurley CA, Jin T, Johnson A, Johnson T, Kenny JR, Koehler MF, Bir Kohli P, Kulagowski JJ, Labadie S, Liao J, Liimatta M, Lin Z, Lupardus PJ, Maxey RJ, Murray JM, Pulk R, Rodriguez M, Savage S, Shia S, Steffek M, Ubhayakar S, Ultsch M, van Abbema A, Ward SI, Xiao L, Xiao Y.
J Med Chem (2012) 55 : 6176 -6193
PubMed 22698084 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 23 6.72 7.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1789941 RUXOLITINIB 4.0 1 7.62
CHEMBL2159143 1 7.58
CHEMBL2159142 1 7.46
CHEMBL221959 TOFACITINIB 4.0 1 7.28
CHEMBL2159202 1 7.24
CHEMBL2159210 1 7.06
CHEMBL2159198 1 7.00
CHEMBL2159205 1 6.96
CHEMBL2159214 1 6.75
CHEMBL2159215 1 6.72
CHEMBL2159141 1 6.60
CHEMBL2159212 1 6.60
CHEMBL2159209 1 6.57
CHEMBL2159208 1 6.55
CHEMBL2159211 1 6.51
CHEMBL2159204 1 6.46
CHEMBL2159200 1 6.46
CHEMBL2159203 1 6.31
CHEMBL2159206 1 6.05
CHEMBL2159199 1 6.00
CHEMBL2159207 1 5.44
CHEMBL2159201 1 -
CHEMBL2159213 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1789941 RUXOLITINIB EC50 = 24.0 nM 7.62
CHEMBL2159143 EC50 = 26.0 nM 7.58
CHEMBL2159142 EC50 = 35.0 nM 7.46
CHEMBL221959 TOFACITINIB EC50 = 53.0 nM 7.28
CHEMBL2159202 EC50 = 57.0 nM 7.24
CHEMBL2159210 EC50 = 87.0 nM 7.06
CHEMBL2159198 EC50 = 100.0 nM 7.00
CHEMBL2159205 EC50 = 110.0 nM 6.96
CHEMBL2159214 EC50 = 180.0 nM 6.75
CHEMBL2159215 EC50 = 190.0 nM 6.72
CHEMBL2159141 EC50 = 250.0 nM 6.60
CHEMBL2159212 EC50 = 250.0 nM 6.60
CHEMBL2159209 EC50 = 270.0 nM 6.57
CHEMBL2159208 EC50 = 280.0 nM 6.55
CHEMBL2159211 EC50 = 310.0 nM 6.51
CHEMBL2159204 EC50 = 350.0 nM 6.46
CHEMBL2159200 EC50 = 350.0 nM 6.46
CHEMBL2159203 EC50 = 490.0 nM 6.31
CHEMBL2159206 EC50 = 890.0 nM 6.05
CHEMBL2159199 EC50 = 990.0 nM 6.00
CHEMBL2159207 EC50 = 3600.0 nM 5.44
CHEMBL2159201 EC50 - -
CHEMBL2159213 EC50 - -