Assay Detail
Binding
CHEMBL2162544
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Escherichia coli recombinant His6-tagged DXR expressed in Escherichia coli XL-1 blue using DXP as substrate incubated for 2 mins prior to substrate addition measured after 5 mins by spectrophotometric analysis
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Modifications around the hydroxamic acid chelating group of fosmidomycin, an inhibitor of the metalloenzyme 1-deoxyxylulose 5-phosphate reductoisomerase (DXR).
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 7 | 7.41 | 7.50 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL203125 | FOSMIDOMYCIN | 3.0 | 1 | 7.50 |
| CHEMBL258981 | — | — | 1 | 7.32 |
| CHEMBL2159155 | — | — | 1 | - |
| CHEMBL2159154 | — | — | 1 | - |
| CHEMBL2159153 | — | — | 1 | - |
| CHEMBL2159149 | — | — | 1 | - |
| CHEMBL440285 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL203125 | FOSMIDOMYCIN | IC50 | = | 32.0 | nM | 7.50 |
| CHEMBL258981 | — | IC50 | = | 48.0 | nM | 7.32 |
| CHEMBL2159155 | — | IC50 | = | 3800000.0 | nM | - |
| CHEMBL2159154 | — | IC50 | = | 930000.0 | nM | - |
| CHEMBL2159153 | — | IC50 | = | 1000000.0 | nM | - |
| CHEMBL2159149 | — | IC50 | = | 2700000.0 | nM | - |
| CHEMBL440285 | — | IC50 | = | 1200000.0 | nM | - |